| IED ID | IndEnz0005000914 |
| Enzyme Type ID | lipase000914 |
| Protein Name |
3-ketodihydrosphingosine reductase TSC10 EC 1.1.1.102 3-dehydrosphinganine reductase KDS reductase Temperature-sensitive CSG2 suppressor protein 10 |
| Gene Name | TSC10 YBR265W YBR1734 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKFTLEDQVVLITGGSQGLGKEFAKKYYNEAENTKIIIVSRSEARLLDTCNEIRIEAHLRRETTDEGQVQHKLAAPLDLEQRLFYYPCDLSCYESVECLFNALRDLDLLPTQTLCCAGGAVPKLFRGLSGHELNLGMDINYKTTLNVAHQIALAEQTKEHHLIIFSSATALYPFVGYSQYAPAKAAIKSLVAILRQELTNFRISCVYPGNFESEGFTVEQLTKPEITKLIEGPSDAIPCKQACDIIAKSLARGDDDVFTDFVGWMIMGMDLGLTAKKSRFVPLQWIFGVLSNILVVPFYMVGCSWYIRKWFRENDGKKAN |
| Enzyme Length | 320 |
| Uniprot Accession Number | P38342 |
| Absorption | |
| Active Site | ACT_SITE 16; /note=Nucleophile; for lipase activity; /evidence=ECO:0000250|UniProtKB:P40471; ACT_SITE 180; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10001 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=NADP(+) + sphinganine = 3-oxosphinganine + H(+) + NADPH; Xref=Rhea:RHEA:22640, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:57817, ChEBI:CHEBI:58299, ChEBI:CHEBI:58349; EC=1.1.1.102; Evidence={ECO:0000269|PubMed:9804843}; |
| DNA Binding | |
| EC Number | 1.1.1.102 |
| Enzyme Function | FUNCTION: Catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS). {ECO:0000269|PubMed:9804843}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Lipid metabolism; sphingolipid metabolism. {ECO:0000269|PubMed:9804843}. |
| nucleotide Binding | NP_BIND 11..19; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 73..75; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 180..184; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506; NP_BIND 211..213; /note=NAD; /evidence=ECO:0000250|UniProtKB:Q92506 |
| Features | Active site (2); Chain (1); Motif (1); Nucleotide binding (4); Sequence conflict (2); Transmembrane (1) |
| Keywords | Endoplasmic reticulum;Lipid metabolism;Membrane;NAD;NADP;Oxidoreductase;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10366774; 11283351; 11805826; 12452424; 12484746; 12785717; 12827281; 14690591; 15070751; 16375675; 16429126; 16730802; 16787378; 16997623; 17449104; 18296751; 18467557; 19141869; 19536198; 19579220; 19854949; 21119626; 21603271; 22909030; 23545423; 24220500; 24520995; 24868093; 25750665; 26357016; 26703187; 26726837; 27664439; 9885152; |
| Motif | MOTIF 14..18; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:P40471 |
| Gene Encoded By | |
| Mass | 35,973 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22640 |
| Cross Reference Brenda |