| IED ID | IndEnz0005000928 |
| Enzyme Type ID | lipase000928 |
| Protein Name |
Putative methylesterase 14, chloroplastic AtMES14 EC 3.1.1.- |
| Gene Name | MES14 At1g33990 F12G12.19 F12G12.220 T15K4.4 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGNKIISMMKKDSKDGGGGGSKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQRFDGSMSRRVGSTSTRKRTLSDPFSNGKQVPDFSESLIVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIAQIP |
| Enzyme Length | 348 |
| Uniprot Accession Number | Q9FVW3 |
| Absorption | |
| Active Site | ACT_SITE 172; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 299; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 327; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Putative methylesterase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (2); Chain (1); Erroneous gene model prediction (1); Modified residue (1); Region (2); Sequence conflict (1); Transit peptide (1) |
| Keywords | Alternative splicing;Chloroplast;Hydrolase;Phosphoprotein;Plastid;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}. |
| Modified Residue | MOD_RES 77; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:19376835 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17317660; 17644812; 18643994; 18650403; 20736450; |
| Motif | |
| Gene Encoded By | |
| Mass | 38,855 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |