| IED ID | IndEnz0005000937 |
| Enzyme Type ID | lipase000937 |
| Protein Name |
Phospholipase A 1 DAD1, chloroplastic EC 3.1.1.32 Phospholipase A1-Ibeta1 Protein DEFECTIVE IN ANTHER DEHISCENCE 1 AtDAD1 |
| Gene Name | DAD1 At2g44810 T13E15.18 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MRFSLSPVRPHSVVVPSLPKQDVVSYISGTTSNRQCRCVLTLPSPSVSTSRPPVLPKPETWESLLLNHDQIPGEFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERSGLPNSGYRLTKNLRATSGINLPRWIEKAPSWMATQSSWIGYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLYTSGVHSLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYDIKTTFKRAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRRVEETPWVYAEIGKELRLSSRDSPHLSSINVATCHELKTYLHLVDGFVSSTCPFRETARRVLHR |
| Enzyme Length | 447 |
| Uniprot Accession Number | Q948R1 |
| Absorption | |
| Active Site | ACT_SITE 295; /note=Acyl-ester intermediate; /evidence=ECO:0000305|PubMed:19527719; ACT_SITE 352; /note=Charge relay system; /evidence=ECO:0000305|PubMed:19527719; ACT_SITE 418; /note=Charge relay system; /evidence=ECO:0000305|PubMed:19527719 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000269|PubMed:11595796, ECO:0000269|PubMed:19527719};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18690; Evidence={ECO:0000269|PubMed:11595796, ECO:0000269|PubMed:19527719}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:40971, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:73002, ChEBI:CHEBI:76084; Evidence={ECO:0000269|PubMed:11595796};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40972; Evidence={ECO:0000269|PubMed:11595796}; |
| DNA Binding | |
| EC Number | 3.1.1.32 |
| Enzyme Function | FUNCTION: Sn-1-specific phospholipase that releases free fatty acids from phospholipids. Low activity on galactolipids and triacylglycerols. Catalyzes the initial step of jasmonic acid biosynthesis. Not essential for jasmonate biosynthesis after wounding or upon pathogen infection. {ECO:0000269|PubMed:11595796, ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:20348210}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0-6.5. {ECO:0000269|PubMed:11595796}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous initiation (4); Motif (1); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Lipid degradation;Lipid metabolism;Plastid;Reference proteome;Transit peptide |
| Interact With | |
| Induction | INDUCTION: Induced by wounding (PubMed:11595796, PubMed:18267087, PubMed:20348210, PubMed:24430866). Induced by methyl jasmonate (PubMed:24430866). {ECO:0000269|PubMed:11595796, ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:20348210, ECO:0000269|PubMed:24430866}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000269|PubMed:11595796, ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:19527719}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 293..297; /note=GXSXG; /evidence=ECO:0000305|PubMed:19527719 |
| Gene Encoded By | |
| Mass | 49,960 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18689; RHEA:18690; RHEA:40971; RHEA:40972 |
| Cross Reference Brenda | 3.1.1.32; |