Detail Information for IndEnz0005000948
IED ID IndEnz0005000948
Enzyme Type ID lipase000948
Protein Name Phospholipase YtpA
EC 3.1.1.-
Bacilysocin biosynthesis protein YtpA
Gene Name ytpA BSU30510
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MWTWKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRSFQEYIDEVDAWIDKARTFDLPVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQIKVNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEADQNDSLYVRKVSVRWYRELLKTIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFNEPEREDVFKAARAFTDQYI
Enzyme Length 259
Uniprot Accession Number O34705
Absorption
Active Site ACT_SITE 88; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 206; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 236; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis reaction of one acyl bond in phosphatidylcholine in vitro (actual cleavage point is unknown), resulting in lysophosphatidylcholine. {ECO:0000269|PubMed:11796336}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Antibiotic biosynthesis; bacilysocin biosynthesis. {ECO:0000269|PubMed:11796336}.
nucleotide Binding
Features Active site (3); Chain (1)
Keywords Antibiotic biosynthesis;Hydrolase;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 29,566
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda