| IED ID | IndEnz0005000949 |
| Enzyme Type ID | lipase000949 |
| Protein Name |
Triacylglycerol lipase 2 EC 3.1.1.3 Lipase 2 Neutral lipid hydrolase |
| Gene Name | TGL2 YDR058C D4225 YD9609.12C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKNDNKANDIIIDSVKVPDSYKPPKNPIVFCHGLSGFDKLILIPSVFHLTNLISNSIVHNMAENFMQDDEDKSDNKYTNLLEIEYWIGVKKFLQSKGCTVITTKVPGFGSIEERAMALDAQLQKEVKKIESKDKRHSLNLIAHSMGGLDCRYLICNIKNRNYDILSLTTISTPHRGSEMADYVVDLFENLNALRVSQKILPICFYQLTTAYMKYFNLVTPNSPKVSYFSYGCSFVPKWYNVFCTPWKIVYERSKGCPNDGLVTINSSKWGEYRGTLKDMDHLDVINWKNKLQDDWSKFFRTTTVGEKVDILNFYLKITDDLARKGF |
| Enzyme Length | 326 |
| Uniprot Accession Number | P54857 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000269|PubMed:9544243}; CATALYTIC ACTIVITY: Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; Evidence={ECO:0000269|PubMed:19959834};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; Evidence={ECO:0000305|PubMed:19959834}; CATALYTIC ACTIVITY: Reaction=1,2,3-tributanoylglycerol + H2O = butanoate + dibutanoylglycerol + H(+); Xref=Rhea:RHEA:40475, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:35020, ChEBI:CHEBI:76478; Evidence={ECO:0000269|PubMed:19959834};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40476; Evidence={ECO:0000305|PubMed:19959834}; CATALYTIC ACTIVITY: Reaction=1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + H(+) + octanoate; Xref=Rhea:RHEA:47864, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:76978, ChEBI:CHEBI:88066; Evidence={ECO:0000269|PubMed:19959834, ECO:0000269|PubMed:28119444};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47865; Evidence={ECO:0000305|PubMed:19959834, ECO:0000305|PubMed:28119444}; CATALYTIC ACTIVITY: Reaction=di-(9Z)-octadecenoylglycerol + H2O = (9Z)-octadecenoate + (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:47868, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:75937, ChEBI:CHEBI:75945; Evidence={ECO:0000269|PubMed:19959834};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47869; Evidence={ECO:0000305|PubMed:19959834}; CATALYTIC ACTIVITY: Reaction=dioctanoylglycerol + H2O = H(+) + octanoate + octanoylglycerol; Xref=Rhea:RHEA:47880, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:88066, ChEBI:CHEBI:88070; Evidence={ECO:0000269|PubMed:19959834, ECO:0000269|PubMed:28119444};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47881; Evidence={ECO:0000305|PubMed:19959834, ECO:0000305|PubMed:28119444}; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Mitochondrial triacylglycerol (TAG) lipase with activity toward long-chain diacylglycerols (DAGs) and triacylglycerols (TAGs) (PubMed:9544243, PubMed:19959834). Involved in mitochondrial lipid metabolism (PubMed:31483742). {ECO:0000269|PubMed:19959834, ECO:0000269|PubMed:31483742, ECO:0000269|PubMed:9544243}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:9544243}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Motif (1); Sequence conflict (2) |
| Keywords | Hydrolase;Lipid degradation;Lipid metabolism;Mitochondrion;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:19959834}. Mitochondrion intermembrane space {ECO:0000269|PubMed:31483742}. Note=Imported into the IMS via the MIA40 disulfide relay system. {ECO:0000269|PubMed:31483742}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18258205; 18422427; 22345606; 23139841; 23275493; 23383298; 24597968; 24678285; 25063300; 26950140; |
| Motif | MOTIF 142..146; /note=(A/G)XSXG lipase motif; /evidence=ECO:0000305|Ref.7 |
| Gene Encoded By | |
| Mass | 37,500 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044; RHEA:38575; RHEA:38576; RHEA:40475; RHEA:40476; RHEA:47864; RHEA:47865; RHEA:47868; RHEA:47869; RHEA:47880; RHEA:47881 |
| Cross Reference Brenda |