| IED ID | IndEnz0005000980 |
| Enzyme Type ID | lipase000980 |
| Protein Name |
Lipid droplet-associated hydrolase EC 3.1.1.- Lipid droplet-associated serine hydrolase |
| Gene Name | LDAH RCJMB04_2g19 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MRSVSEEPVPLHEEFIYCCGAATHVLKCGPWKDLSKDESKNLPRLLFMIIPGNPGLAGYYRTFIKALYCGLNQQYPVWVVSHAGHCKPPSGMEMIEDTDIKELEDVFGLNGQVEHKLNFLKKNVSKDIKLVLIAHSIGCYITLEMMKRASELQVLRSVLLFPTIERMAQSPQGKLMTPLLCKLRYALYMPVYLLSFLPEGVKASLVRFALRGMKTCDESSITTSVNLFSVDCIANILYMASQEMMKVVERDSTTIKQNLKKLIFYYGTGDSWCPQNYYDEIKMDFPDGDIRLCEKGLRHAFVLDASKEMAAMITDWLRDDLTKL |
| Enzyme Length | 324 |
| Uniprot Accession Number | Q5F477 |
| Absorption | |
| Active Site | ACT_SITE 136; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 270; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9; ACT_SITE 299; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Probable serine lipid hydrolase associated with lipid droplets. Appears to lack cholesterol esterase activity. Appears to lack triglyceride lipase activity. {ECO:0000250|UniProtKB:Q8BVA5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous initiation (1) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid droplet;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q8BVA5}. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q8BVA5}. Note=Localizes to the endoplasmic reticulum in absence of lipid droplets and translocates to lipid droplets upon lipid storage induction. {ECO:0000250|UniProtKB:Q8BVA5}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 36,882 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |