| IED ID | IndEnz0005001033 |
| Enzyme Type ID | lipase001033 |
| Protein Name |
Putative lipase ATG15 EC 3.1.1.3 Autophagy-related protein 15 |
| Gene Name | ATG15 HCAG_08049 |
| Organism | Ajellomyces capsulatus (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Ajellomycetaceae Histoplasma Ajellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum) Ajellomyces capsulatus (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum) |
| Enzyme Sequence | MMGLDLLVSLLALSVSPCIAATRSPLQIPVLDTQPIETGREFTLRHMLHHGTYRDPLLHKRLDIRPDTLLWAASENGEKRESVPRFRVSSRPINIHRLSDRHVSVVEDYLSIARMTSSAVTLSSDYWTLDEVDAPDVTDKETVLSLAKMTSNAYIMIPGSGEWFDVVPPFNHSDSFGWDSDGLRGHIYSDNTNSTIVVVLKGTSAAIFDGGGTTTNDKVNDNLFFSCCCAQGGHYFWRQVCDCYSSTYTCNTACVRKALRQENRYYRAALNLYSNITAMYPQSNIWVTGHSLGGAVSSLLGMTYGLPVVTFEAVPEALPASRLGLPPPPGTDPSSPQARNYTGAYHFGHTADPIYMGTCNGATSVCTLGGYAMESSCHTGQLCTYDTVEDFGWRVGIGTHRIREVITDVIERYDDVPTCAPFTECVDCNNWKFFESNETTPTPTTTTTSTSTRTRTSTCKTPGWWGCLDPTTTPTTTATTSTTSTSTCKTPGWFGCKDPTTTSTVPTASPAPTITPTSPPTTTASTTSTCESPGWFGCNDPTSTTTFPVPSTTSTSTPCSTPGWFWGCRDQTTTTSASPPITSPP |
| Enzyme Length | 585 |
| Uniprot Accession Number | A6REI4 |
| Absorption | |
| Active Site | ACT_SITE 291; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (1); Glycosylation (5); Region (1); Topological domain (1); Transmembrane (1) |
| Keywords | Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 63,488 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |