Detail Information for IndEnz0005001041
IED ID IndEnz0005001041
Enzyme Type ID lipase001041
Protein Name Putative lipase atg15
EC 3.1.1.3
Autophagy-related protein 15
Gene Name atg15 CHGG_05687
Organism Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Chaetomiaceae Chaetomium Chaetomium globosum (Soil fungus) Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus)
Enzyme Sequence MSIGEVSDSAGHLASLVLPIEVAPIAPLIPEPPATAEHIFTLRHVYHHGTHKHPSLHRKHDVTPSNADVWLAAEDGYEAERIGPLRARSNALRIQRLVDRRPSVVDPMVAQSRQQGFVSVLSPSAWTVDDVAGPDVTDKGTVLTMALMAANAYVEGPGKADWQDVGAPFNRSLDFGWEGDGLRGHVFADENNSTIVIGLKGTSPAVFDGDGTTTNDKVNDNLFFSCCCAQQGPWTWHQVCDCATGTYTCNNTCVTQALREENRYYQAARELYANVTEVYPDSHVWIAGHSLGGAVSSFLGLTYGVPVVTFQAVPDALPAGRLGLPVPPGADPNAPQSRDYTGAFHFGHTADPIYMGSCNGATASCSFAGYALESACHTGHECVYDTVGDKGWRVGIGTHKIVAVIRDVILKYDTVPECKFTPECRDCGNWKMYESNGTETTTTSSAPTTTSISRTRTETCKTPHLKSQQQRQRPRPRPLHALPQWEGGNPDSPERNEEM
Enzyme Length 499
Uniprot Accession Number Q2H6M8
Absorption
Active Site ACT_SITE 290; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by atg15 is critical to life span extension (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Glycosylation (6); Region (1); Topological domain (2); Transmembrane (1)
Keywords Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,141
Kinetics
Metal Binding
Rhea ID RHEA:12044
Cross Reference Brenda