Detail Information for IndEnz0005001044
IED ID IndEnz0005001044
Enzyme Type ID lipase001044
Protein Name Putative lipase ATG15
EC 3.1.1.3
Autophagy-related protein 15
Gene Name ATG15 CNC01140
Organism Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Tremellales (jelly fungi) Cryptococcaceae Cryptococcus Cryptococcus neoformans species complex Cryptococcus neoformans (Filobasidiella neoformans) Cryptococcus neoformans var. neoformans Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Enzyme Sequence MYIPGPLRLSSYLLPFLSSPSPPAQSSPDTRTISFKPVHAHGHAFVDNASTPTLLFLDQSPSASLYAHDYPIGAFGDDYVLPRLTSDVLEIRTRKKLIRRPKVRPPRIISWAQSYRAQALHFNGINNNNDNSNKSISLPESWLAPDLANPSDEWSDVEVTVPDLTDRQTVITLAKMSSNAYVTPGGAGWYTLNDWNASMPFGWEPDADGLRGHVFADEKNETVIISIKGTSAGVLGSGGPTAKNDKFNDNLLFSCCCARVDFSWTPVCDCYAGGYKCGQTCLEDALVSESVYATVGTNLYNNITYMYPNATIWLTGHSLGGAVSSLIGLSFGAPAVTYESPGELLPASRLHLPLPPGMPANLSGITHVYHTADPIAMGVCNGPYSSCYAAGFAMESKCHTGETILYDTVRVKGWSVDVRTHRIEEVIDKVLADPWPEEGEGKSGVWEKAVEGWYRAADRVRAALDESVVRDDVNVWWGWGRRGPKRQPGGEDPGWRKHGGVPKPVSEEDCVDCYKWEFGEWN
Enzyme Length 522
Uniprot Accession Number P0CO60
Absorption
Active Site ACT_SITE 318; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Glycosylation (7); Region (1); Topological domain (2); Transmembrane (1)
Keywords Autophagy;Endoplasmic reticulum;Endosome;Glycoprotein;Golgi apparatus;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Endosome, multivesicular body membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Prevacuolar compartment membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 57,210
Kinetics
Metal Binding
Rhea ID RHEA:12044
Cross Reference Brenda