| IED ID | IndEnz0005001047 |
| Enzyme Type ID | lipase001047 |
| Protein Name |
Putative lipase ATG15 EC 3.1.1.3 Autophagy-related protein 15 |
| Gene Name | ATG15 FG02519 FGRAMPH1_01T06045 |
| Organism | Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium Fusarium sambucinum species complex Gibberella zeae (Wheat head blight fungus) (Fusarium graminearum) Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) |
| Enzyme Sequence | MYKYGTVVDPAMTTNRRSRLSGFRCASTARVTATLLLSFLAFSPSSASSDFGDRFHDDIVLPPGPVLPSAPEIPEPAEHTFSLRHIYHHGTHLHPSLHRKRDVVHEQSRVYLAAEDDFSEYDILRLKAKSRPEPIHRLADRRPSVVDPMVAESRQRGYAAVLDASAWTMDQVSSPDIKDKDTVLTLALMTANAYVEHDTDADWEDVGERWNRSADFGWESDGLRGHVFVDDTNSTIVIGLKGTTTAVFDGEGTTTNDKVNDNLFFSCCCAQQGQWTWHQVCDCATGTYSCNNTCVVQALREENRYYGAARELYSNVTELYPDAQVWLTGHSLGGAVTSMLGMTYGLPVVTFEAVPEALPASRLGLPVPPGASAEYPQMRENTGTFHFGHTADPVYIGTCNGATASCTYGGYAMESTCHAGYECVYDVVADKGWRVGIGTHRIRSVIDDVIKKYDGVPECKRTPECRDCAQWKMYESNGTETTTTSSPSTTSTTRTRTRTSTCETPGWWGCLDKTTPVTTTTSTTSSSTSTCKTPGWFGCKDKTTSESSTTTTTEASPTTTCETPGRFWGCRDEVEATTTAKPGQVTNVPVTAAPTGTTDDSLSTALPETQRCLARNWFGICKEWAIDDLEFATDEM |
| Enzyme Length | 636 |
| Uniprot Accession Number | I1RFN8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000250|UniProtKB:P25641}; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies (By similarity). Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles (By similarity). The intravacuolar membrane disintegration by ATG15 is critical to life span extension (By similarity). Autophagy is required for proper vegetative growth, asexual/sexual reproduction, and full virulence (PubMed:28894236). Autophagy is particularly involved in the biosynthesis of deoxynivalenol (DON), an important virulence determinant (PubMed:28894236). {ECO:0000250|UniProtKB:P25641, ECO:0000269|PubMed:28894236}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (5); Region (1); Topological domain (2); Transmembrane (1) |
| Keywords | Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 69,735 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |