Detail Information for IndEnz0005001048
IED ID IndEnz0005001048
Enzyme Type ID lipase001048
Protein Name Putative lipase ATG15
EC 3.1.1.3
Autophagy-related protein 15
Gene Name ATG15 KLLA0C08679g
Organism Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Kluyveromyces Kluyveromyces lactis (Yeast) (Candida sphaerica) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Enzyme Sequence MKPGIKISKRYSARNASVITVLLLLIYLIYINKETIQTKYQGSRINHDSGGDSTKDKTHTFTIKEIHFRPLDEKTQSYGSLQVTPEFVNMAKSEFEQNVAVASGDNEDLYDKQLWSASIKSNPWTHQFTLKQGSIKMKRMVNRDPDYVESFLDYAHENPEMARKVHLDWVDESVLAPNVTDKETVISLALMSSNAYVRLPYEGDWRNLSDWNNDLNPDLSVGIGWDSDGVRGHIFSNDDSSVIVIALKGTSAQGLPGSGEDETTDNDKLNDNVLFSCCCARVSYLWKTACDCYVKSYTCDEKCLEQELVRKDRYYQAVLDIYRSVVTAHPNSAIWITGHSLGGALASLLGRTFGAPAVAFEAPGELLATKRLHLPMPPGLPAYQEGVWHIGHTADPIFMGTCNGASSSCSIAGYAMETSCHSGKVCVYDVVTDKGWHVNMLNHRIHTVIDGILTDYDTVAKCKTPDACHDCFNWNYVKGRDVPKKHKSSSSTASSTSAETSTLTVGPSPPEKTTTSCIGRNWIGICTEYGI
Enzyme Length 531
Uniprot Accession Number Q6CU02
Absorption
Active Site ACT_SITE 340; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Glycosylation (2); Region (1); Topological domain (2); Transmembrane (1)
Keywords Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 58,819
Kinetics
Metal Binding
Rhea ID RHEA:12044
Cross Reference Brenda