Detail Information for IndEnz0005001052
IED ID IndEnz0005001052
Enzyme Type ID lipase001052
Protein Name Putative lipase ATG15
EC 3.1.1.3
Autophagy-related protein 15
Gene Name ATG15 pSI-7
Organism Passalora fulva (Tomato leaf mold) (Cladosporium fulvum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta dothideomyceta Dothideomycetes Dothideomycetidae Mycosphaerellales Mycosphaerellaceae Passalora Passalora fulva (Tomato leaf mold) (Cladosporium fulvum)
Enzyme Sequence MIWNGRLVLACVLLIAGCSGQVDAARTREQRKAFGSPNIVLPPGPAHEVASAKPARALGEKTFTLRHVYHHGTHNYPNLHRYIDIQPESKLQVSYDDGTTYEEAEVAFKAKAQSRIVQRMAHRSHSDIDAVQVHYFTYGQPADVEWTEDEIAGPNISDKSTVLTFAKMAANAYIFSRKDGEWQPVKGGFNYTDDFGWEQDGLRGHIFADEANSTVVIGLKGTSPAIFDGADTTGNDKLNDNLFGSCCCAQGGPFTWKKVCDCAGSSAYTCNNTCLVKSLREKGHYYWAVKDLYRNVTERYPDSEVWLSGHSLGGVVSSLLGLTYGLPTLTFEAFPDAMAASRLGLPTPPGYKIGSHQARPMTGIHHFGHTADPIYMGSCNGGTSFCSIGGYAFEGVCHTGETCTYDTVRDLGWRVGIGTHKIVSVIKDVIKVYDQPPSCEQDVECVDCYNWKYFENNGTETTTSKASTATSTTTRTRTETCKTPGWWGCLDESTTSAPTTSTSTSSSTSSTRTCETPGWFGCKDETTTTSSSSSISSPSATPAPTITTTSSLPTSTSTSTCKTPGWFGCYDESTTTSATPKPSSTARTVTKTKTTTSDDDDCTSREWFGLICVDPSPTTASGSSPSSTNLPTTRRKMCTKRHWYGTCKQWRFDDFDPKNDL
Enzyme Length 661
Uniprot Accession Number O13444
Absorption
Active Site ACT_SITE 311; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Glycosylation (6); Region (2); Topological domain (2); Transmembrane (1)
Keywords Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction INDUCTION: During starvation and plant infection. {ECO:0000269|PubMed:9390425}.
Subcellular Location SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 71,955
Kinetics
Metal Binding
Rhea ID RHEA:12044
Cross Reference Brenda