Detail Information for IndEnz0005001053
IED ID IndEnz0005001053
Enzyme Type ID lipase001053
Protein Name Putative lipase atg15
EC 3.1.1.3
Autophagy-related protein 15
Gene Name atg15 Pc22g21230
Organism Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium chrysogenum species complex Penicillium rubens Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Enzyme Sequence MPRKRSRFELSIHSLLLSVAVLSGAAYASGYYPPSQQVPIIPPQVPLKGAEATPDIHEFSLRHIFHRGTYEQPDFHARLDVKPDTRLRTVSEDGHEEEYIASESSLLASSSPLTIQRLADRRLPVIQGHLAAARSSGFAAALSPQEWALDTLPGPNITDKPTVLTFAQMTANDYIEEPGTGQWHTINGKFNYSGSFGWQKDGLRGHIYSDKTNGTVVISLKGTSPALFDGAGTTTNDKVNDNLYFSCCCGQGGSYLWRQSCDCQSATFTANLTCIIESMTDEDRYYRAAIDLYSNVTEIYPDANIWMTGHSLGGAMTSLVGLTFGLPVVTFEAVPEALPAARLGLPSPPGYDPRFPQSRQYTGAYHFGHTADPVFMGTCNGINSICTWGGYAMESVCHTGQVCTYDTVADKGWRVGLGTHKIENVISDVLMTYDSVPSCVAEEECFDCELWKFFRSNGSEITTTTTTTTTTTSPTRTSTCKTPGWWGCLDESTTATTTTTTTTTSTATTTTCMTPGWFGCNDPTTTTATPAPTVTTTLPTVTSTTTSCHDPGWFGCRDETSTVATTTANPASPTSTACHSPGIFWGCWDEPTSTTAVTSLPAFTSAPISTTCHSPGIFWGCWDEPTSTTAVTSLPAITSTPISTTCHIPGIFWGCWDEPTNTPAVTSAPTPTS
Enzyme Length 673
Uniprot Accession Number A7KAM5
Absorption
Active Site ACT_SITE 311; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by atg15 is critical to life span extension (By similarity). {ECO:0000250, ECO:0000269|PubMed:17204848}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Glycosylation (6); Topological domain (2); Transmembrane (1)
Keywords Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 72,414
Kinetics
Metal Binding
Rhea ID RHEA:12044
Cross Reference Brenda