| IED ID | IndEnz0005001054 |
| Enzyme Type ID | lipase001054 |
| Protein Name |
Putative lipase ATG15 EC 3.1.1.3 Autophagy-related protein 15 |
| Gene Name | ATG15 |
| Organism | Pichia angusta (Yeast) (Hansenula polymorpha) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Pichiaceae Ogataea Pichia angusta (Yeast) (Hansenula polymorpha) |
| Enzyme Sequence | MGNPTLMRWPVTRILVLGLVVTVLYKAMAIYSSRRAPVQVCFTSPSSAFKIKHIFHNAADGRTHKRLDIDDEFLSIYNSARSQMAHIEDMDLFTVSPFEQELVIRHTEGQTVRLADRSPDFVESYLDYALENGPASRMIEFDWQQESLLIPNMSDKETIVSLALMSSDAYVGLPTDADWNDVGDKYNESERFGWENGGVRGHVFTDPEETIVIISIKGTSAAGLNTGGDGQTVEQDKTNDNLLFSCCCARVSSLWKTVCDCYEESYTCNQNCLEQELRRPDRYYKAVLEIYRNVTHMYPESEIWVTGHSLGGSLSSLLGRTFGLPAVSFEAVGELLATRRLHLPMPPGLPEEMENIWHVGNTADPIFMGVCNGASSTCSLAGYALETQCHSGKKCVYDVVNDLGWHVNLLNHRIRTIISDVLMVYNDTAECVKPPPCYDCYNWRFVDHSGDRYRTTASSPSPIPSDPPGRRCLKRTWYGRCYEWADDPSAVKTTFATSTIYRKTSDISTSTHTGGNTTCIRRSWLGYCLEYGPELR |
| Enzyme Length | 536 |
| Uniprot Accession Number | A7KAJ9 |
| Absorption | |
| Active Site | ACT_SITE 309; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension (By similarity). {ECO:0000250, ECO:0000269|PubMed:17204848}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (5); Topological domain (2); Transmembrane (1) |
| Keywords | Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Prevacuolar compartment membrane {ECO:0000250|UniProtKB:P25641}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P25641}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000250|UniProtKB:P25641}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,260 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |