| IED ID | IndEnz0005001062 |
| Enzyme Type ID | lipase001062 |
| Protein Name |
Putative lipase ATG15 EC 3.1.1.3 Autophagy-related protein 15 Cytoplasm to vacuole targeting protein 17 |
| Gene Name | ATG15 AUT5 CVT17 YCR068W YCR68W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MLHKSPSRKRFASPLHLGCILTLTVLCLIAYYFALPDYLSVGKSSSRGAMDQKSDGTFRLKSIYRHGVGANHRLHQRLEVTPEVISAAGMLYQETTTQGQDFEDQEPLWTTNAEYATTNPFDFEFELRRMPLLMKRMKERDPEFIESYIYGETYMTEEEEHAMWIDDDIVAPNITDRGTVVSLALMSSNAYVRIPQTGDWRNVTEPWNETEPEDFGWDGDGIRGHVFYNEVENIVVLSIKGTSAQGLPGSGEDETTGNDKINDNLLFSCCCARVSYLWTTVCDCYVKSYICDESCLEKELRRKDRFYSAVVDIYKGVLKEYPDAAIWVTGHSLGGALASLLGRTFGLPAVAFESPGELLPSKRLHLPFPPGLPSYMEGIWHFGHNADPIFMGTCNGASSSCSLVGYAMETACHTGRVCVYDVVNDKGWSVNMFNHRIHKVIDEVLLGYEQAAKCVEPEPCVDCYNWKFIPSRDWESSSRLITKTKSHAAPTTTTRTTATTTSSSTCVGRNWLGFCTKYEL |
| Enzyme Length | 520 |
| Uniprot Accession Number | P25641 |
| Absorption | |
| Active Site | ACT_SITE 332; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension. {ECO:0000269|PubMed:11085977, ECO:0000269|PubMed:11264288, ECO:0000269|PubMed:11566994, ECO:0000269|PubMed:12499386, ECO:0000269|PubMed:18690010, ECO:0000269|PubMed:21364763, ECO:0000269|PubMed:21777356}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (3); Mutagenesis (1); Topological domain (2); Transmembrane (1) |
| Keywords | Autophagy;Endosome;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endosome, multivesicular body membrane {ECO:0000269|PubMed:12499386}; Single-pass type II membrane protein {ECO:0000269|PubMed:12499386}. Prevacuolar compartment membrane {ECO:0000269|PubMed:12499386}; Single-pass type II membrane protein {ECO:0000269|PubMed:12499386}. Note=From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC). {ECO:0000269|PubMed:12499386}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:11085977, ECO:0000269|PubMed:11566994, ECO:0000269|PubMed:12499386}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10735854; 11099404; 11169858; 11283351; 11528006; 12503998; 12586695; 15350980; 15615779; 16093310; 16247502; 16534619; 16860663; 17224625; 18006683; 18701704; 18719252; 18806213; 19010432; 19250605; 19619494; 19653858; 19744312; 20083110; 20124347; 20146925; 20157579; 20223289; 20487284; 20712412; 21194379; 21315770; 21317551; 21548784; 21846491; 21912624; 22150273; 22480867; 22489171; 22550491; 22743996; 22768199; 22992453; 23202731; 23416930; 23669359; 23733851; 23750326; 23821148; 24040173; 24258026; 24366340; 24789822; 25187651; 25426852; 26061644; 26081468; 26108642; 26344037; 26490118; 27050453; 27189367; 27543826; 33625870; 8663607; |
| Motif | |
| Gene Encoded By | |
| Mass | 58,435 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |