| IED ID | IndEnz0005001064 |
| Enzyme Type ID | lipase001064 |
| Protein Name |
Autophagy-related protein 18 |
| Gene Name | atg-18 atgr-18 tag-283 F41E6.13 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MSATTSEENPDSINYIGFNQDSKVICVGHKDGYMFYKTADILENNTLTYEGENLTHLGLNNCLIIERLFSSALMVVISQKDPRVLHVYHFTSRNIICDHRFNKSVLTVRLNRDRIVVCLEDCIYIYNLKDMKMMHNIMDTPTNKLGVLDLTSNPGNALIAYPGSTDTGSVHLFDAINLSSVSTFNAHEGTIACLKFNQEGNMIATASTKGTVIRVYSVPNGHRLFEFRRGVTRCVNIYSLCFSSDSKYLTSSSNTETVHVFKLEKTEGVDNKPEASTEGGGWFDAINKTFSAYMPSQVLQVGELMTTERSFATAKLPGAARSNQVSLVSHKNQQYVMAATSDGFVYAYRLDPEGGELDLIKQHNIGPKSDTSRASPTSTGSGGAAKSAEASNQSVPNMDDPDDFPPMSHTSG |
| Enzyme Length | 412 |
| Uniprot Accession Number | O16466 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Component of the autophagy machinery that is recruited to phosphatidylinositols on preautophagosomal structures, which are early autophagic structures, to promote autophagosome formation, and the subsequent degradation and clearance of engulfed apoptotic cells and P-granules in somatic cells (PubMed:12958363, PubMed:19167332, PubMed:21183797, PubMed:21802374, PubMed:22451698, PubMed:25124690, PubMed:28557996). In particular, binds with high affinity to phosphatidylinositols including phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidylinositol 5-phosphate (PtdIns(5)P), and more weakly to phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PubMed:21802374, PubMed:22451698). Plays a role in mitophagy, which is the autophagic consumption of mitochondria, in response to dietary restriction (PubMed:30133321). Involved in xenophagy, the autophagy-mediated degradation of pathogens and pathogen products, such as toxins (PubMed:27875098). Also plays a role in membrane-pore repair (PubMed:27875098). In a daf-18/PTEN- and daf-16/FOXO-dependent manner, required for the proliferation of germ stem cell progenitors in the gonad during the late phases of larval development (PubMed:28285998). By regulating the release of neurotransmitters and neuropeptides, involved in the control of lifespan in response to dietary restriction and daf-2 signaling (PubMed:28557996). Probably through its involvement in autophagy, required for dauer formation (PubMed:12958363). {ECO:0000269|PubMed:12958363, ECO:0000269|PubMed:19167332, ECO:0000269|PubMed:21183797, ECO:0000269|PubMed:21802374, ECO:0000269|PubMed:22451698, ECO:0000269|PubMed:25124690, ECO:0000269|PubMed:27875098, ECO:0000269|PubMed:28285998, ECO:0000269|PubMed:28557996, ECO:0000269|PubMed:30133321, ECO:0000303|PubMed:22451698}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Chain (1); Compositional bias (1); Motif (1); Mutagenesis (2); Region (1); Repeat (4) |
| Keywords | Alternative splicing;Autophagy;Cytoplasm;Cytoplasmic vesicle;Lipid-binding;Membrane;Reference proteome;Repeat;WD repeat |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome membrane {ECO:0000269|PubMed:22451698}; Peripheral membrane protein {ECO:0000305|PubMed:22451698}; Cytoplasmic side {ECO:0000305|PubMed:22451698}. Cytoplasm {ECO:0000269|PubMed:25124690}. Note=Partially localizes to the phagosome membrane of engulfed apoptotic cells. {ECO:0000269|PubMed:22451698}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14551910; 15338614; 16530747; 17164286; 17172799; 17417969; 17486083; 17850180; 17901876; 19343510; 20382828; 20537990; 21177967; 21285529; 21502138; 21906946; 21998252; 22347378; 22560298; 22718972; 22862955; 23108454; 23530068; 23800452; 24165672; 25487147; 26759963; 27208080; 29348603; 29748380; 30871075; |
| Motif | MOTIF 227..230; /note="L/FRRG motif"; /evidence="ECO:0000269|PubMed:21802374, ECO:0000269|PubMed:22451698" |
| Gene Encoded By | |
| Mass | 45,274 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |