| IED ID | IndEnz0005001107 |
| Enzyme Type ID | lipase001107 |
| Protein Name |
Phospholipase A1 EG1, chloroplastic/mitochondrial EC 3.1.1.32 Protein EXTRA GLUME 1 |
| Gene Name | EG1 Os01g0900400 LOC_Os01g67430 P0035F12.8 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MTLPRQCAAACRTGGGGGGVVRCRAVAAAGGAVAVRDAVVAPVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAAADVSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNRAAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLATYISLLRGADKKQPAAAAADAGGVLAKVMDFVGRRRGGGALPWHDAAMIQMGGLVQTLGLI |
| Enzyme Length | 435 |
| Uniprot Accession Number | Q8S1D9 |
| Absorption | |
| Active Site | ACT_SITE 266; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 324; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 371; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000305|PubMed:27367609}; |
| DNA Binding | |
| EC Number | 3.1.1.32 |
| Enzyme Function | FUNCTION: Phospholipase that releases free fatty acids from phospholipids. Catalyzes the initial step of jasmonate (JA) biosynthesis. Required for the biosynthesis of endogenous JA in seedling, inflorescence and spikelets. Not essential for JA biosynthesis after wounding (PubMed:24647160). Mediates spikelet development and specification of empty-glume identity (PubMed:18980657, PubMed:24647160). Functions in a high temperature-dependent manner to maintain floral developmental robustness under heat stress conditions. Functions by safeguarding the expression of several floral identity genes, such as MADS1, MADS6 and G1 (PubMed:27367609). {ECO:0000269|PubMed:18980657, ECO:0000269|PubMed:24647160, ECO:0000269|PubMed:27367609}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous initiation (2); Motif (1); Mutagenesis (2); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Lipid degradation;Lipid metabolism;Mitochondrion;Plastid;Reference proteome;Stress response;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By high temperature. {ECO:0000269|PubMed:27367609}. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:27367609}. Plastid, chloroplast {ECO:0000269|PubMed:24647160}. Note=Localizes predominantly in mitochondrion. {ECO:0000269|PubMed:27367609}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 264..268; /note=GXSXG; /evidence=ECO:0000305|PubMed:24647160 |
| Gene Encoded By | |
| Mass | 46,011 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18689 |
| Cross Reference Brenda |