| IED ID | IndEnz0005001111 |
| Enzyme Type ID | lipase001111 |
| Protein Name |
Carboxylesterase 5A EC 3.1.1.1 Carboxylesterase-like urinary excreted protein Cauxin |
| Gene Name | CES5A CES7 |
| Organism | Felis catus (Cat) (Felis silvestris catus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Carnivora Feliformia Felidae (cat family) Felinae Felis Felis catus (Cat) (Felis silvestris catus) |
| Enzyme Sequence | MSGMWVHPGRTLIWALWVLAAVIKGPAADAPVRSTRLGWVRGKQTTVLGSTVPVNMFLGIPYAAPPLGPLRFKQPKPALPGNDFRNATSYPKLCFQDLEWLVSYQHVLKVRYPKLEASEDCLYLNIYAPAHADNGSNLPVMVWFPGGAFKMGSASSFDGSALAAYEDVLIVTTQYRLGIFGFFDTGDEHARGNWALLDQVAALTWVRDNIEFFGGDPRSVTIFGESAGAISVSSLILSPIANGLFHKAIMESGVAILPLLMRPPGDERKKDLQVLARICGCHASDSAALLQCLRAKPSEELMDISKKLTFSIPVIDDFFFPDEPVALLTQKAFNSVPSIIGVNNHECAFLLSTEFSEILGGSNRSLALYLVHTFLNIPTQYLHLVADHYFYNKHSPVEIRDSFLDLLGDVLFVVPGVVTARYHRDAGAPVYFYEFQHPPQCLNDTRPAFVKADHSDEIRFVFGGAFLKGDIVMFEGATEEEKLLSRKMMRYWANFARTGDPNGEGVPLWPAYTQSEQYLKLDLSVSVGQKLKEQEVEFWMNTIVP |
| Enzyme Length | 545 |
| Uniprot Accession Number | Q8I034 |
| Absorption | |
| Active Site | ACT_SITE 226; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 346; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 454; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | FUNCTION: Carboxylesterase present at high level in urine that regulates production of felinine, a probable pheromone precursor. Probably acts by hydrolyzing the peptide bond of the felinine precursor 3-methylbutanol cyteinylglycine, producing felinine and glycine in cat urine. {ECO:0000269|PubMed:17052611}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (4); Signal peptide (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | INDUCTION: Strongly down-regulated in cats suffering from tubulointerstitial nephritis (TIN). Excretion decreases immediately after castration. {ECO:0000269|PubMed:16919690, ECO:0000269|PubMed:17045831}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated; contains a fucosylated complex carbohydrate. {ECO:0000269|PubMed:12401131, ECO:0000269|PubMed:17341822}. |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000269|PubMed:12401131 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,506 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=506 uM for p-NPA {ECO:0000269|PubMed:12401131}; Vmax=7.61 umol/min/mg enzyme with p-NPA as substrate {ECO:0000269|PubMed:12401131}; |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |