| IED ID | IndEnz0005001114 |
| Enzyme Type ID | lipase001114 |
| Protein Name |
Homer protein homolog 1 PSD-Zip45 VASP/Ena-related gene up-regulated during seizure and LTP 1 Vesl-1 |
| Gene Name | Homer1 Homer Vesl |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MGEQPIFSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPAQNALPFSHSAGDRTQGLSHASSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHSHKTELSQTVQELEETLKVKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSELEQRLEKSQSEQDAFRSNLKTLLEILDGKIFELTELRDNLAKLLECS |
| Enzyme Length | 366 |
| Uniprot Accession Number | Q9Z214 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 3 counteracts the effects by competing for binding sites. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, and isoform 2 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (PubMed:19345194). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (By similarity). {ECO:0000250|UniProtKB:Q86YM7, ECO:0000269|PubMed:14528310, ECO:0000269|PubMed:19345194}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (3); Beta strand (7); Chain (1); Coiled coil (1); Compositional bias (2); Domain (1); Erroneous initiation (1); Helix (2); Initiator methionine (1); Modified residue (2); Mutagenesis (12); Region (2); Sequence conflict (1); Turn (3) |
| Keywords | 3D-structure;Acetylation;Alternative splicing;Cell junction;Cell projection;Coiled coil;Cytoplasm;Phosphoprotein;Reference proteome;Synapse |
| Interact With | Q3UVX5; Q8CGU4; Q14CM0; Q3UVX5; Itself; Q9WV48; Q3UVX5 |
| Induction | INDUCTION: [Isoform 3]: Induced in the hippocampus, by seizure and synaptic mechanisms in association with long-term potentiation (LTP). It is also induced in the striatum by drugs that alter dopamine signaling. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell junction, synapse, postsynaptic density {ECO:0000250}. Cell junction, synapse {ECO:0000269|PubMed:10433269}. Cell projection, dendritic spine {ECO:0000269|PubMed:19345194, ECO:0000269|PubMed:30021165}. Note=Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. {ECO:0000250}. |
| Modified Residue | MOD_RES 2; /note=N-acetylglycine; /evidence=ECO:0000250|UniProtKB:Q86YM7; MOD_RES 318; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9Z2Y3 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1DDV; 1DDW; 1I2H; 3CVE; |
| Mapped Pubmed ID | 12867517; 15308308; 15758184; 15813924; 16554037; 17880892; 18932227; 19105975; 19547699; 21217644; 23911326; 29267967; 9647694; |
| Motif | |
| Gene Encoded By | |
| Mass | 41,305 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |