Detail Information for IndEnz0005001117
IED ID IndEnz0005001117
Enzyme Type ID lipase001117
Protein Name Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1
EC 3.1.1.n2
Isoprenylcysteine methylesterase-like protein 1
Gene Name ICMEL1 At1g26120 F14G11.9 F28B23.20
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MPSQILQISHHLPPKSSPSTEMMFKSLIYDDPSTTLLSRFGDDHHTISSTVKPLLSRSSSYNGTAMKTSSSSSAGGFTGWYQNRRRRSNSDNCLSAFSDDTNGTADGGNNSGDRQTTIGQEVGHAAAETFLLTRLCLKLLSYLGVGYRWITRFMALGCYAFLLMPGFIQVGYYYFFSPYVRRSIVYGDQPRNRLDLYLPKNSTGPKPVVAFVTGGAWIIGYKAWGSLLGQQLSERDIIVACIDYRNFPQGSISDMVKDASSGISFVCNHIAEYGGDPDRIYLMGQSAGAHIAACTIVEQVIKESGEGDSVSWSSSQINAYFGLSGGYNLLNLVDHFHSRGLYRSIFLSIMEGEESLRQFSPELVVQNPNLKHIIARLPPFILFHGTDDYSIPSDASKSFAETLQRLGAKAKVILYEGKTHTDLFLQDPMRGGIDEMFEDIVTVVLGDDQEAIGKSVDRRRLVPEFMLKLAHWVSPF
Enzyme Length 476
Uniprot Accession Number Q8VYP9
Absorption
Active Site ACT_SITE 286; /evidence=ECO:0000305; ACT_SITE 388; /evidence=ECO:0000305; ACT_SITE 420; /evidence=ECO:0000305
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125, Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2; Evidence={ECO:0000250|UniProtKB:Q94AS5};
DNA Binding
EC Number 3.1.1.n2
Enzyme Function FUNCTION: Catalyzes the demethylation of isoprenylcysteine methylesters (By similarity). May be involved in the regulation of ABA signaling (By similarity). {ECO:0000250|UniProtKB:Q94AS5}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Alternative sequence (1); Chain (1); Erroneous gene model prediction (2); Region (3); Transmembrane (2)
Keywords Alternative splicing;Endoplasmic reticulum;Golgi apparatus;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction INDUCTION: Down-regulated by heat treatment. {ECO:0000269|PubMed:20868530}.
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:20868530}. Golgi apparatus membrane {ECO:0000269|PubMed:20868530}; Multi-pass membrane protein {ECO:0000269|PubMed:20868530}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 14738307; 16870359; 16895613;
Motif
Gene Encoded By
Mass 52,719
Kinetics
Metal Binding
Rhea ID RHEA:48520
Cross Reference Brenda