IED ID | IndEnz0005001118 |
Enzyme Type ID | lipase001118 |
Protein Name |
Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL2 EC 3.1.1.n2 Isoprenylcysteine methylesterase-like protein 2 |
Gene Name | ICMEL2 At3g02410 F16B3.4 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MQLSPERCRPMSENREAWSANSEEMELLHGSNRLSSPEHVRRRVSGNSSEDGSPRICRQQSFGRDIGHAAAETYLITRLSFNLLGYLGVGYRWITRLLALACYAMLLMPGFLQVAYLYFFSSQVRRSIVYGGHPRNRLDLYIPPTSDGLKPVVVFVTGGAWIIGYKAWGSLLGLQLAERDIIVACLDYRNFPQGTISDMVSDAAQGISFVCNNISAFGGDPNRIYLMGQSAGAHISSCALFEQAIKESRGESISWSVSQIKAYFGLSGGYNLFNLVEHFHNRGLYRSIFLSIMEGEESFKQFSPEVRLKDLNVRKAAALLPHIILFHGSADYSIPPEASKTFTDALQAAEVKAELVMYKGKTHTDLFLQDPLRGGKDELFDHIVSMIHADDSDALRNDAVAPPRKRLVPEFLLKLAGRVSPF |
Enzyme Length | 422 |
Uniprot Accession Number | Q1PET6 |
Absorption | |
Active Site | ACT_SITE 230; /evidence=ECO:0000305; ACT_SITE 331; /evidence=ECO:0000305; ACT_SITE 363; /evidence=ECO:0000305 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125, Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2; Evidence={ECO:0000250|UniProtKB:Q94AS5}; |
DNA Binding | |
EC Number | 3.1.1.n2 |
Enzyme Function | FUNCTION: Catalyzes the demethylation of isoprenylcysteine methylesters (By similarity). May act as a negative regulator of ABA signaling (PubMed:20868530). {ECO:0000250|UniProtKB:Q94AS5, ECO:0000269|PubMed:20868530}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Alternative sequence (2); Chain (1); Erroneous gene model prediction (1); Erroneous termination (1); Region (3); Transmembrane (2) |
Keywords | Alternative splicing;Endoplasmic reticulum;Golgi apparatus;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:20868530}. Golgi apparatus membrane {ECO:0000269|PubMed:20868530}; Multi-pass membrane protein {ECO:0000269|PubMed:20868530}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 14576160; 14738307; 16626458; 16870359; 16895613; 20032078; |
Motif | |
Gene Encoded By | |
Mass | 47,010 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:48520 |
Cross Reference Brenda |