| IED ID | IndEnz0005001126 |
| Enzyme Type ID | lipase001126 |
| Protein Name |
Phospholipase A 1 LCAT3 EC 3.1.1.32 Lecithin-cholesterol acyltransferase-like 3 |
| Gene Name | LCAT3 At3g03310 T21P5.27 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGWIPCPCWGTNDDENAGEVADRDPVLLVSGIGGSILHSKKKNSKSEIRVWVRIFLANLAFKQSLWSLYNPKTGYTEPLDDNIEVLVPDDDHGLYAIDILDPSWFVKLCHLTEVYHFHDMIEMLVGCGYKKGTTLFGYGYDFRQSNRIDLLILGLKKKLETAYKRSGGRKVTIISHSMGGLMVSCFMYLHPEAFSKYVNKWITIATPFQGAPGCINDSILTGVQFVEGLESFFFVSRWTMHQLLVECPSIYEMMANPDFKWKKQPEIRVWRKKSENDVDTSVELESFGLIESIDLFNDALKNNELSYGGNKIALPFNFAILDWAAKTREILNKAQLPDGVSFYNIYGVSLNTPFDVCYGTETSPIDDLSEICQTMPEYTYVDGDGTVPAESAAAAQFKAVASVGVSGSHRGLLRDERVFELIQQWLGVEPKKAKRKHLRTHKVVDSG |
| Enzyme Length | 447 |
| Uniprot Accession Number | Q93V61 |
| Absorption | |
| Active Site | ACT_SITE 177; /note=Acyl-ester intermediate; /evidence=ECO:0000305; ACT_SITE 384; /note=Charge relay system; /evidence=ECO:0000305; ACT_SITE 409; /note=Charge relay system; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; |
| DNA Binding | |
| EC Number | 3.1.1.32 |
| Enzyme Function | FUNCTION: Hydrolyzes the sn-1 acylester bond of phospholipids. Phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid can be used as substrates. Weak activity with lysophosphatidylcholine and no activity with tripalmitoylglycerol and cholesteryl oleate. Seems to have a preference for unsaturated fatty acids at the sn-1 position. {ECO:0000269|PubMed:15355352}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 to 65 degrees Celsius. {ECO:0000269|PubMed:15355352}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0-6.5. {ECO:0000269|PubMed:15355352}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous gene model prediction (1); Mutagenesis (5) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Microsome;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Microsome membrane {ECO:0000269|PubMed:15355352}; Peripheral membrane protein {ECO:0000269|PubMed:15355352}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15247387; 16020547; 23505340; |
| Motif | |
| Gene Encoded By | |
| Mass | 50,349 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18689 |
| Cross Reference Brenda | 3.1.1.32; |