| IED ID | IndEnz0005001158 |
| Enzyme Type ID | lipase001158 |
| Protein Name |
Juvenile hormone esterase JHE EC 3.1.1.59 |
| Gene Name | Tcjhe TcasGA2_TC013193 |
| Organism | Tribolium castaneum (Red flour beetle) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Tenebrionoidea Tenebrionidae (darkling ground beetles) Tenebrionidae incertae sedis Tribolium Tribolium castaneum (Red flour beetle) |
| Enzyme Sequence | MKFPKNLFLVLFYTSWKFCDVCAYTPSHPLVYTKYGSVIGSVEYSRNSRAYMSFKGIPFAKPPVGDLRFKAPEPPEPWNFSINGTKDAPFCIQKNYFFSNPKVEGSEDCLYLNVYVPKTEGSQLLPVMVFIHWGGFFAGRGSSDYIGPEYIMDKDVILVTFNYRLGVFGFLSTLDDNAPGNFGLKDQVMALKFVHENIECFGGDNNRVTIFGQSAGSGSVNLHLISPASRGLFQQAISQSGAALDLWARPLNALQPNVTAALAAFTGCSAHIGSSKDIVDCLRKIEATKLAETADNFKYFSIEPLTPYSMVTEKQTDANPNPFLVQDPLESLKAGAFMKIPWMVGSVQDEGILRAAPLIRQPETLQTLNSNFEKLITQMLFLQFSAGANASSLLKNMTDFYLGGKSLIDVNNPKSVQGFINLYGDRAFHYGIYQTVILQLRKGHKPIWMYNFNYKGQYSYGDKFAATDKNVNFTWGVSHCDDLLYLFKSPGLFANLQKDNDILMSKTMVSFWTNFAIYGNPDPHQNLNWNSLNFEKPEGVKVADLNIMHITGNHETGKIAFDVEKSQILDRIAFWAKQSLLENFPDFG |
| Enzyme Length | 588 |
| Uniprot Accession Number | D6WMZ8 |
| Absorption | |
| Active Site | ACT_SITE 214; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 350; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23141; ACT_SITE 479; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23141 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by 3-octylthio-1,1,1-trifluoro-2-propanone (OTFP), a specific inhibitor of juvenile hormone esterase (JHE), but not by diisopropyl fluorophosphate (DFP), a serine enzyme inhibitor. {ECO:0000269|PubMed:20522120}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + juvenile hormone III = H(+) + juvenile hormone III carboxylate + methanol; Xref=Rhea:RHEA:46912, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:27493, ChEBI:CHEBI:83274; EC=3.1.1.59; Evidence={ECO:0000269|PubMed:20522120}; |
| DNA Binding | |
| EC Number | 3.1.1.59 |
| Enzyme Function | FUNCTION: May function as a juvenile hormone (JH)-specific degradation enzyme in vivo decreasing JH activity. Hydrolyzes JH III in vitro. Hydrolyzes effectively also methyl hepthylthioacetothioate (HEPTAT), a synthetic substrate. Of the general esterase substrates, it has preference for 2-naphthyl acetate (2-NA) and shows a weak activity for 1-NA and 4-nitrophenylacetate (4-NPA). {ECO:0000269|PubMed:20522120}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (6); Sequence conflict (10); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20522120}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 65,853 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=657 nM for juvenile hormone (JH) III {ECO:0000269|PubMed:20522120}; Note=kcat is 0.164 sec(-1) for JH III. {ECO:0000269|PubMed:20522120}; |
| Metal Binding | |
| Rhea ID | RHEA:46912 |
| Cross Reference Brenda | 3.1.1.59; |