| IED ID | IndEnz0005001161 |
| Enzyme Type ID | lipase001161 |
| Protein Name |
Phospholipid--sterol O-acyltransferase EC 2.3.1.- Lecithin-cholesterol acyltransferase-like 2 |
| Gene Name | PSAT LCAT2 PSAT1 At1g04010 F21M11.5 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGANSKSVTASFTVIAVFFLICGGRTAVEDETEFHGDYSKLSGIIIPGFASTQLRAWSILDCPYTPLDFNPLDLVWLDTTKLLSAVNCWFKCMVLDPYNQTDHPECKSRPDSGLSAITELDPGYITGPLSTVWKEWLKWCVEFGIEANAIVAVPYDWRLSPTKLEERDLYFHKLKLTFETALKLRGGPSIVFAHSMGNNVFRYFLEWLRLEIAPKHYLKWLDQHIHAYFAVGAPLLGSVEAIKSTLSGVTFGLPVSEGTARLLSNSFASSLWLMPFSKNCKGDNTFWTHFSGGAAKKDKRVYHCDEEEYQSKYSGWPTNIINIEIPSTSVTETALVNMTSMECGLPTLLSFTARELADGTLFKAIEDYDPDSKRMLHQLKKLYHDDPVFNPLTPWERPPIKNVFCIYGAHLKTEVGYYFAPSGKPYPDNWIITDIIYETEGSLVSRSGTVVDGNAGPITGDETVPYHSLSWCKNWLGPKVNITMAPQPEHDGSDVHVELNVDHEHGSDIIANMTKAPRVKYITFYEDSESIPGKRTAVWELDKTNHRNIVRSPVLMRELWLQMWHDIQPGAKSKFVTKAKRGPLRDADCYWDYGKACCAWQEYCEYRYSFGDVHLGQSCRLRNTSANMLLQYI |
| Enzyme Length | 633 |
| Uniprot Accession Number | Q4VCM1 |
| Absorption | |
| Active Site | ACT_SITE 195; /note=Acyl-ester intermediate; /evidence=ECO:0000255; ACT_SITE 461; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 505; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Involved in lipid catabolism. Essential for sterol esters biosynthesis in leaves and seeds, but not in flowers. Plays a role in controlling the free sterol content of leaves. Catalyzes the transacylation of acyl groups from phospholipids to a variety of different sterols. Prefers phosphatidylethanolamine over phosphatidylcholine as an acyl donor. Not active toward neutral lipids. Highly specific for position sn-2, which in plant lipids is essentially devoid of saturated acyl groups. Broad sterol specificity (cholesterol > campesterol > sitosterol > stigmasterol), but no activity with lupeol or beta-amyrin. {ECO:0000269|PubMed:16020547, ECO:0000269|PubMed:19923239}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous gene model prediction (1); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Acyltransferase;Endoplasmic reticulum;Lipid metabolism;Membrane;Microsome;Reference proteome;Signal-anchor;Steroid metabolism;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: By senescence. {ECO:0000269|PubMed:19923239}. |
| Subcellular Location | SUBCELLULAR LOCATION: Microsome membrane {ECO:0000269|PubMed:16020547}; Single-pass type II membrane protein {ECO:0000269|PubMed:16020547}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12566579; 15247387; 15355352; 23072470; 23505340; 30034403; 31712757; 32185672; |
| Motif | |
| Gene Encoded By | |
| Mass | 71,688 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |