| IED ID | IndEnz0005001165 |
| Enzyme Type ID | lipase001165 |
| Protein Name |
Lipid droplet-associated hydrolase EC 3.1.1.- Lipid droplet-associated serine hydrolase |
| Gene Name | DDB_G0286581 |
| Organism | Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
| Enzyme Sequence | MELIEEIINLDEAIQGETRTEIIYTKSQTPSNIKIIVIAGNPGIESFYQEFVKVLNLSFNSKYDIYGVGHIGHCGKIENKTFSVEEQIKHKELFLEYLLKNKYGDKDRKDIKFILIGHSVGSYISLKVVSRFSEKFEFLSVVNLFPTFKNLYDGLSPFIKMVVMRESTRNGLSTFLHYIPSIVVSNVLKWILPSDESRIAVQSKINYYSALNILYMAYTETEDIKEIDDECHSVFNSRLNQLLFIYGQTDSYTPKSFYDEMKQLYPAGNIEYSSSYVPHAFVLHHSQEVALRVSEWLSLNILKN |
| Enzyme Length | 304 |
| Uniprot Accession Number | Q54LL8 |
| Absorption | |
| Active Site | ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 250; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9; ACT_SITE 279; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Probable serine lipid hydrolase associated with lipid droplets. Appears to lack cholesterol esterase activity. Appears to lack triglyceride lipase activity. {ECO:0000250|UniProtKB:Q8BVA5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Hydrolase;Lipid droplet;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q8BVA5}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 22120990; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,128 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |