| IED ID | IndEnz0005001167 |
| Enzyme Type ID | lipase001167 |
| Protein Name |
Lipase EC 3.1.1.3 Triacylglycerol lipase |
| Gene Name | |
| Organism | Rhizomucor miehei |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Mucoromycota Mucoromycotina Mucoromycetes Mucorales (pin molds) Lichtheimiaceae Rhizomucor Rhizomucor miehei |
| Enzyme Sequence | MVLKQRANYLGFLIVFFTAFLVEAVPIKRQSNSTVDSLPPLIPSRTSAPSSSPSTTDPEAPAMSRNGPLPSDVETKYGMALNATSYPDSVVQAMSIDGGIRAATSQEINELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIKTWSTLIYDTNAMVARGDSEKTIYIVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQNELVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYRRTVNERDIVPHLPPAAFGFLHAGEEYWITDNSPETVQVCTSDLETSDCSNSIVPFTSVLDHLSYFGINTGLCT |
| Enzyme Length | 363 |
| Uniprot Accession Number | P19515 |
| Absorption | |
| Active Site | ACT_SITE 238; /note=Nucleophile; /evidence=ECO:0000305|PubMed:2304552; ACT_SITE 297; /note=Charge relay system; /evidence=ECO:0000305|PubMed:2304552; ACT_SITE 351; /note=Charge relay system; /evidence=ECO:0000305|PubMed:2304552 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Compositional bias (1); Disulfide bond (3); Helix (12); Metal binding (1); Propeptide (1); Region (1); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Calcium;Disulfide bond;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24 |
| Structure 3D | X-ray crystallography (6) |
| Cross Reference PDB | 1TGL; 3TGL; 4TGL; 5TGL; 6QPP; 6QPR; |
| Mapped Pubmed ID | 31460089; |
| Motif | |
| Gene Encoded By | |
| Mass | 39,602 |
| Kinetics | |
| Metal Binding | METAL 350; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda | 3.1.1.3; |