IED Industry Enzyme Database

Detail Information for IndEnz0005001230
IED ID IndEnz0005001230
Enzyme Type ID lipase001230
Protein Name Bile salt-activated lipase
BAL
EC 3.1.1.13
EC 3.1.1.3
EC 3.1.1.6
Bile salt-stimulated lipase
BSSL
Carboxyl ester lipase
Cholesterol esterase
Pancreatic lysophospholipase
Sterol esterase
Gene Name Cel
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MGRLEVLFLGLTCCLAAACAAKLGAVYTEGGFVEGVNKKLSLLGGDSVDIFKGIPFATAKTLENPQRHPGWQGTLKATDFKKRCLQATITQDDTYGQEDCLYLNIWVPQGRKQVSHDLPVMVWIYGGAFLMGSGQGANFLKNYLYDGEEIATRGNVIVVTFNYRVGPLGFLSTGDANLPGNFGLRDQHMAIAWVKRNIAAFGGDPDNITIFGESAGAASVSLQTLSPYNKGLIRRAISQSGVALSPWAIQENPLFWAKTIAKKVGCPTEDTAKMAGCLKITDPRALTLAYRLPLKSQEYPIVHYLAFIPVVDGDFIPDDPINLYDNAADIDYLAGINDMDGHLFATVDVPAIDKAKQDVTEEDFYRLVSGHTVAKGLKGTQATFDIYTESWAQDPSQENMKKTVVAFETDILFLIPTEMALAQHRAHAKSAKTYSYLFSHPSRMPIYPKWMGADHADDLQYVFGKPFATPLGYRAQDRTVSKAMIAYWTNFAKSGDPNMGNSPVPTHWYPYTTENGNYLDINKKITSTSMKEHLREKFLKFWAVTFEMLPTVVGDHTPPEDDSEAAPVPPTDDSQGGPVPPTDDSQTTPVPPTDNSQAGDSVEAQMPGPIGF
Enzyme Length 612
Uniprot Accession Number P07882
Absorption
Active Site ACT_SITE 214; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 340; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 455; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation ACTIVITY REGULATION: Activated by bile salts such as sodium taurocholate. {ECO:0000250|UniProtKB:P19835}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + H(+) + octanoate; Xref=Rhea:RHEA:47864, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:76978, ChEBI:CHEBI:88066; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47865; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10101; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, ChEBI:CHEBI:46898; Evidence={ECO:0000269|PubMed:1999399, ECO:0000269|PubMed:2211595};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; Evidence={ECO:0000305|PubMed:1999399}; CATALYTIC ACTIVITY: Reaction=an acetyl ester + H2O = acetate + an aliphatic alcohol + H(+); Xref=Rhea:RHEA:12957, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:47622; EC=3.1.1.6; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12958; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+); Xref=Rhea:RHEA:47348, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:50477; Evidence={ECO:0000269|PubMed:1999399, ECO:0000269|PubMed:2211595};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47349; Evidence={ECO:0000305|PubMed:1999399}; CATALYTIC ACTIVITY: Reaction=9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + H(+) + hexadecanoate; Xref=Rhea:RHEA:52052, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:83670, ChEBI:CHEBI:136286; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52053; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=9-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-octadecenoate + 9-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52048, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:136282, ChEBI:CHEBI:136286; Evidence={ECO:0000250|UniProtKB:P19835};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52049; Evidence={ECO:0000250|UniProtKB:P19835}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) + hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:72998; Evidence={ECO:0000269|PubMed:2211595};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436; Evidence={ECO:0000305|PubMed:2211595}; CATALYTIC ACTIVITY: Reaction=12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + H(+) + hexadecanoate; Xref=Rhea:RHEA:52056, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:83677, ChEBI:CHEBI:84201; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52057; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=12-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-octadecenoate + 12-hydroxyoctadecanoate + H(+); Xref=Rhea:RHEA:52060, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:84201, ChEBI:CHEBI:136302; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52061; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=13-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-octadecenoate + 13-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52064, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:136303, ChEBI:CHEBI:136304; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52065; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52068, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:136286, ChEBI:CHEBI:136309; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52069; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 12-hydroxyoctadecanoate + H(+); Xref=Rhea:RHEA:52072, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:84201, ChEBI:CHEBI:136312; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52073; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 13-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52076, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:136304, ChEBI:CHEBI:136315; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52077; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52080, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:84201, ChEBI:CHEBI:136330; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52081; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52084, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:136304, ChEBI:CHEBI:136335; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52085; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=5-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 5-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52092, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:136369, ChEBI:CHEBI:136370; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52093; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52096, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:136286, ChEBI:CHEBI:136373; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52097; Evidence={ECO:0000250|UniProtKB:Q64285};
DNA Binding
EC Number 3.1.1.13; 3.1.1.3; 3.1.1.6
Enzyme Function FUNCTION: Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFA) (PubMed:2211595, PubMed:1999399). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (By similarity). Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins (By similarity). {ECO:0000250|UniProtKB:P19835, ECO:0000250|UniProtKB:Q64285, ECO:0000269|PubMed:1999399, ECO:0000269|PubMed:2211595}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Compositional bias (1); Disulfide bond (2); Glycosylation (1); Mutagenesis (3); Region (2); Repeat (4); Sequence conflict (10); Signal peptide (1)
Keywords Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Repeat;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P19835}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10811659; 11239821; 15265857; 15296737; 17805199; 2775770; 8446851; 9295161; 9701565;
Motif
Gene Encoded By
Mass 67,040
Kinetics
Metal Binding
Rhea ID RHEA:12044; RHEA:12045; RHEA:38575; RHEA:38576; RHEA:47864; RHEA:47865; RHEA:10100; RHEA:10101; RHEA:33875; RHEA:33876; RHEA:12957; RHEA:12958; RHEA:47348; RHEA:47349; RHEA:52052; RHEA:52053; RHEA:52048; RHEA:52049; RHEA:40435; RHEA:40436; RHEA:52056; RHEA:52057; RHEA:52060; RHEA:52061; RHEA:52064; RHEA:52065; RHEA:52068; RHEA:52069; RHEA:52072; RHEA:52073; RHEA:52076; RHEA:52077; RHEA:52080; RHEA:52081; RHEA:52084; RHEA:52085; RHEA:52092; RHEA:52093; RHEA:52096; RHEA:52097
Cross Reference Brenda