| IED ID | IndEnz0005001238 |
| Enzyme Type ID | lipase001238 |
| Protein Name |
Chlorophyllase type 0 EC 3.1.1.14 CaCLH0 Chlorophyll-chlorophyllido hydrolase 0 Chlase 0 |
| Gene Name | CACLH |
| Organism | Chenopodium album (Fat-hen) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae Caryophyllales Chenopodiaceae Chenopodioideae Atripliceae Chenopodium Chenopodium album (Fat-hen) |
| Enzyme Sequence | MAKLLLLIFGVFIFVNSQAQTFPTILEKHNSEKITDVFHKGNFQVTNNPIRVKRYEFSAPEPLIIISPKEAGVYPVLLFIHGTMLSNEDYSLFFNYIASHGFIVVAPKLFRLFPPKLPSQQDEIDMAASVANWMPLYLQVVLQRYVTGVEGDLEKLAISGHSRGGKSAFALALGFSNIKLDVTFSALIGVDPVAGRSVDDRTLPHVLTYKPNSFNLSIPVTVIGSGLGNHTISCAPNHVSHQQFYDECKENSSHFVITKYGHMDMLNEFRLSPIAVTMSLMCAQSFRPKATMRRTLGGIMVAFLNAYFRDDGRQYYAIIANRSLAPTNLFAEKKGFNFGFATTYAQL |
| Enzyme Length | 347 |
| Uniprot Accession Number | Q9LE89 |
| Absorption | |
| Active Site | ACT_SITE 162; /note=Nucleophile; /evidence=ECO:0000305|PubMed:12552153; ACT_SITE 191; /note=Charge relay system; /evidence=ECO:0000305|PubMed:12552153; ACT_SITE 262; /note=Charge relay system; /evidence=ECO:0000305|PubMed:12552153 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF) or p-chloromercuribenzoic acid (PCMB), but not by N-ethylmaleimide (NEM) or iodoacetamide. {ECO:0000269|PubMed:12552153}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol; Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292; EC=3.1.1.14; Evidence={ECO:0000269|PubMed:12552153};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19606; Evidence={ECO:0000269|PubMed:12552153}; CATALYTIC ACTIVITY: Reaction=chlorophyll a + H2O = chlorophyllide a + H(+) + phytol; Xref=Rhea:RHEA:38011, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17327, ChEBI:CHEBI:58416, ChEBI:CHEBI:83348; Evidence={ECO:0000269|PubMed:10611389};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38012; Evidence={ECO:0000269|PubMed:10611389}; |
| DNA Binding | |
| EC Number | 3.1.1.14 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol. {ECO:0000269|PubMed:10611389, ECO:0000269|PubMed:12552153}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Porphyrin-containing compound metabolism; chlorophyll degradation. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (4); Motif (1); Mutagenesis (14); Propeptide (1); Signal peptide (1) |
| Keywords | Chlorophyll catabolism;Direct protein sequencing;Glycoprotein;Hydrolase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 160..164; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:Q948R1 |
| Gene Encoded By | |
| Mass | 38,707 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:19605; RHEA:19606; RHEA:38011; RHEA:38012 |
| Cross Reference Brenda | 3.1.1.14; |