Detail Information for IndEnz0005001240
IED ID IndEnz0005001240
Enzyme Type ID lipase001240
Protein Name Chlorophyllase type 1
EC 3.1.1.14
CaCLH1
Chlorophyll-chlorophyllido hydrolase 1
Chlase 1
Fragments
Gene Name
Organism Chenopodium album (Fat-hen)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae Caryophyllales Chenopodiaceae Chenopodioideae Atripliceae Chenopodium Chenopodium album (Fat-hen)
Enzyme Sequence SEKITVFQKGNFQVTEXSSHFV
Enzyme Length 22
Uniprot Accession Number P59677
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol; Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292; EC=3.1.1.14;
DNA Binding
EC Number 3.1.1.14
Enzyme Function FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.6-8.6 for chlorophyll a hydrolysis, and 6.6-7.6 for chlorophyll b hydrolysis.;
Pathway PATHWAY: Porphyrin-containing compound metabolism; chlorophyll degradation.
nucleotide Binding
Features Chain (1); Non-adjacent residues (1); Non-terminal residue (1); Sequence uncertainty (1)
Keywords Chlorophyll catabolism;Direct protein sequencing;Hydrolase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 2,524
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.0 uM for chlorophyll a; KM=3.1 uM for chlorophyll b;
Metal Binding
Rhea ID RHEA:19605
Cross Reference Brenda