| IED ID | IndEnz0005001244 |
| Enzyme Type ID | lipase001244 |
| Protein Name |
Chlorophyllase type 2 EC 3.1.1.14 CaCLH2 Chlorophyll-chlorophyllido hydrolase 2 Chlase 2 Fragments |
| Gene Name | |
| Organism | Chenopodium album (Fat-hen) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae Caryophyllales Chenopodiaceae Chenopodioideae Atripliceae Chenopodium Chenopodium album (Fat-hen) |
| Enzyme Sequence | SEKITVFQKNFQVTENSSHFV |
| Enzyme Length | 21 |
| Uniprot Accession Number | P59678 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol; Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292; EC=3.1.1.14; |
| DNA Binding | |
| EC Number | 3.1.1.14 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.6-8.6 for chlorophyll a hydrolysis, and 6.6-7.6 for chlorophyll b hydrolysis.; |
| Pathway | PATHWAY: Porphyrin-containing compound metabolism; chlorophyll degradation. |
| nucleotide Binding | |
| Features | Chain (1); Non-adjacent residues (1); Non-terminal residue (1) |
| Keywords | Chlorophyll catabolism;Direct protein sequencing;Hydrolase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,470 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.6 uM for chlorophyll a; KM=4.4 uM for chlorophyll b; |
| Metal Binding | |
| Rhea ID | RHEA:19605 |
| Cross Reference Brenda |