| IED ID | IndEnz0005001263 |
| Enzyme Type ID | lipase001263 |
| Protein Name |
GDSL esterase/lipase EC 3.1.1.- CpEST Extracellular lipase |
| Gene Name | |
| Organism | Carica papaya (Papaya) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Caricaceae Carica Carica papaya (Papaya) |
| Enzyme Sequence | MEKPSGQFLGLSLLLLPLLLPISCNAQQLFIFGDSLYDNGNKPFLATDVPSTFWPYGLSIDFPNGRWSDGRIVPDFIAEFLGIPFPPPVLDRSANFSSGVTFATADATILGTPPQTLTLGDQVKAFAQIKSTWTDAQRQKGIYMFYIGANDYLNYTNANLNATAQQQEAFVSQVIAKLKDQLLAIYGLGGRKFAFQNLAPLGCLPIVKQDFKTGNFCLPLASNLAAQHNQLLSETLENLSETLDGFNYIIYDYFNSSLRRMARPNNYGYFTTNLACCGTGSHDAFGCGFKNVHSNLCSYQRGYMFFDGRHNAEKTNEAVAHLIFSADPSVVFPMNLRELFVHP |
| Enzyme Length | 343 |
| Uniprot Accession Number | P86276 |
| Absorption | |
| Active Site | ACT_SITE 35; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 307; /evidence=ECO:0000250; ACT_SITE 310; /evidence=ECO:0000250|UniProtKB:Q9FLN0 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Has lipolytic activity towards triacylglycerols, vinyl esters and phospholipid. Of the triacylglycerols, the highest activity is towards tributyrin. {ECO:0000269|PubMed:19555778}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8-9. {ECO:0000269|PubMed:19555778}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (5); Sequence conflict (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9FLN0}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,991 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |