| IED ID | IndEnz0005001331 |
| Enzyme Type ID | lipase001331 |
| Protein Name |
GDSL esterase/lipase 1 EC 3.1.1.- Extracellular lipase 1 |
| Gene Name | GLIP1 At5g40990 MEE6.6 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MENSQLVSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFELN |
| Enzyme Length | 374 |
| Uniprot Accession Number | Q9FLN0 |
| Absorption | |
| Active Site | ACT_SITE 44; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q09LX1; ACT_SITE 338; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q09LX1; ACT_SITE 341; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q09LX1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Confers resistance to the necrotrophic fungus Alternaria brassicicola. Possesses lipase and antimicrobial activities that directly disrupt fungal spore integrity. Triggers systemic resistance, mostly by the ethylene-dependent pathway. {ECO:0000269|PubMed:16126835}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Ethylene signaling pathway;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Plant defense;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: By ethylene. {ECO:0000269|PubMed:16126835}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16126835}. Note=Secreted upon salicylic acid treatment. {ECO:0000269|PubMed:16126835}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15235117; 19077166; 19146828; 19296849; 24170202; 24631536; 28840447; 30598046; 31022256; |
| Motif | |
| Gene Encoded By | |
| Mass | 41,706 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |