| IED ID | IndEnz0005001340 |
| Enzyme Type ID | lipase001340 |
| Protein Name |
Gibberellin receptor GID1A EC 3.-.-.- AtCXE10 Carboxylesterase 10 GID1-like protein 1 Protein GA INSENSITIVE DWARF 1A AtGID1A |
| Gene Name | GID1A CXE10 GID1L1 At3g05120 T12H1.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAASDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGESGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC |
| Enzyme Length | 345 |
| Uniprot Accession Number | Q9MAA7 |
| Absorption | |
| Active Site | ACT_SITE 191; /evidence="ECO:0000250|UniProtKB:Q0ZPV7, ECO:0000255|PROSITE-ProRule:PRU10038"; ACT_SITE 289; /evidence="ECO:0000250|UniProtKB:Q0ZPV7, ECO:0000255|PROSITE-ProRule:PRU10038" |
| Activity Regulation | |
| Binding Site | BINDING 116; /note="Gibberellin A3"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSH"; BINDING 127; /note="Gibberellin A3"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSH"; BINDING 127; /note="Gibberellin A4"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSI"; BINDING 191; /note="Gibberellin A3"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSH"; BINDING 191; /note="Gibberellin A4"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSI"; BINDING 238; /note="Gibberellin A3; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSH"; BINDING 320; /note="Gibberellin A3; via amide nitrogen"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSH"; BINDING 320; /note="Gibberellin A4; via amide nitrogen"; /evidence="ECO:0000269|PubMed:19037309, ECO:0007744|PDB:2ZSI" |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.-.-.- |
| Enzyme Function | FUNCTION: Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C. {ECO:0000269|PubMed:16709201, ECO:0000269|PubMed:17194763, ECO:0000269|PubMed:17521411}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (10); Binding site (8); Chain (1); Helix (11); Initiator methionine (1); Modified residue (1); Motif (1); Region (1); Turn (8) |
| Keywords | 3D-structure;Acetylation;Gibberellin signaling pathway;Hydrolase;Nucleus;Receptor;Reference proteome |
| Interact With | Q9SV55; Q9LQT8; Q9STX3; O04294; Q9SG92; Q9SLH3; Q9C8Y3; Q8GXW1; Q9LF53 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. |
| Modified Residue | MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0000250|UniProtKB:Q9LT10 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2ZSH; 2ZSI; |
| Mapped Pubmed ID | 17416730; 17933900; 18216856; 18359847; 18650403; 18827182; 20221582; 20430474; 20736450; 21098733; 21323638; 21478445; 21778177; 21852756; 22523240; 23261263; 23590427; 23765254; 23818171; 23893173; 23982476; 24521922; 24961590; 25104541; 26136598; 26927225; 27899534; 28128230; 29042542; 29080908; 29192140; 29907394; 31245748; 31262665; 31519798; 31960739; |
| Motif | MOTIF 113..115; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Gene Encoded By | |
| Mass | 38,618 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |