| IED ID | IndEnz0005001393 |
| Enzyme Type ID | lipase001393 |
| Protein Name |
3-O-acetylpapaveroxine carboxylesterase CXE1 EC 3.1.1.105 Carboxylesterase 1 |
| Gene Name | CXE1 |
| Organism | Papaver somniferum (Opium poppy) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Ranunculales Papaveraceae Papaveroideae Papaver Papaver somniferum (Opium poppy) |
| Enzyme Sequence | MADPYEFLMCIHNPEEDTLTRNFPIPATPLDQNTKDISLNPDRKTSLRIFRPPTKEPPVTKNKLLPIIIYFHGGGFILFNADSTMNHDFCQSIATHIPALVVSVDYRLAPENRLPAAYDDAVDALNWVKDQGLGKLNNSEVWLKEYGDFSKCFIMGCSSGANVAYHASLRAIEMDLEPAKINGLILHCPFFGSLERTESDSKVINNQDLPLAVRDVMWELALPLGSTRDHVYCNPNIDHDGSSSGNMVGLIERCFVVGFYGDPLIDRQIQLVKMLEEKGVKVETWIEQGGYHGVLCFDPMIRETFLEKLKHFILNDEFIY |
| Enzyme Length | 320 |
| Uniprot Accession Number | I3PLR2 |
| Absorption | |
| Active Site | ACT_SITE 158; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 262; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 292; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=3-O-acetylpapaveroxine + H2O = acetate + H(+) + narcotine hemiacetal; Xref=Rhea:RHEA:57400, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:141645, ChEBI:CHEBI:141667; EC=3.1.1.105; Evidence={ECO:0000269|PubMed:25485687, ECO:0000269|PubMed:27378283};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57401; Evidence={ECO:0000305|PubMed:25485687}; |
| DNA Binding | |
| EC Number | 3.1.1.105 |
| Enzyme Function | FUNCTION: Carboxylesterase involved in the biosynthesis of the benzylisoquinoline alkaloid noscapine (PubMed:25485687, PubMed:27378283, PubMed:29610307). Converts 3-O-acetylpapaveroxine to papaveroxine which spontaneously rearranges to narcotine hemiacetal (PubMed:25485687, PubMed:27378283). {ECO:0000269|PubMed:25485687, ECO:0000269|PubMed:27378283, ECO:0000269|PubMed:29610307}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Alkaloid biosynthesis. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Motif (1) |
| Keywords | Alkaloid metabolism;Hydrolase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 72..74; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Gene Encoded By | |
| Mass | 36,168 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=47 uM for 3-O-acetylpapaveroxine {ECO:0000269|PubMed:25485687}; Vmax=336 nmol/min/mg enzyme with 3-O-acetylpapaveroxine as substrate {ECO:0000269|PubMed:25485687}; |
| Metal Binding | |
| Rhea ID | RHEA:57400; RHEA:57401 |
| Cross Reference Brenda | 3.1.1.105; |