| IED ID | IndEnz0005001465 |
| Enzyme Type ID | lipase001465 |
| Protein Name |
Esterase-6 Est-6 EC 3.1.1.1 Carboxylic-ester hydrolase 6 Carboxylesterase-6 |
| Gene Name | Est-6 EST6 CG6917 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MNYVGLGLIIVLSCLWLGSNASDTDDPLLVQLPQGKLRGRDNGSYYSYESIPYAEPPTGDLRFEAPEPYKQKWSDIFDATKTPVACLQWDQFTPGANKLVGEEDCLTVSVYKPKNSKRNSFPVVAHIHGGAFMFGAAWQNGHENVMREGKFILVKISYRLGPLGFVSTGDRDLPGNYGLKDQRLALKWIKQNIASFGGEPQNVLLVGHSAGGASVHLQMLREDFGQLARAAFSFSGNALDPWVIQKGARGRAFELGRNVGCESAEDSTSLKKCLKSKPASELVTAVRKFLIFSYVPFAPFSPVLEPSDAPDAIITQDPRDVIKSGKFGQVPWAVSYVTEDGGYNAALLLKERKSGIVIDDLNERWLELAPYLLFYRDTKTKKDMDDYSRKIKQEYIGNQRFDIESYSELQRLFTDILFKNSTQESLDLHRKYGKSPAYAYVYDNPAEKGIAQVLANRTDYDFGTVHGDDYFLIFENFVRDVEMRPDEQIISRNFINMLADFASSDNGSLKYGECDFKDNVGSEKFQLLAIYIDGCQNRQHVEFP |
| Enzyme Length | 544 |
| Uniprot Accession Number | P08171 |
| Absorption | |
| Active Site | ACT_SITE 209; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 466; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | FUNCTION: Transferred from the ejaculatory bulbs of males to the female genitals upon copulation, plays an important role in the reproductive biology. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (17); Chain (1); Disulfide bond (3); Erroneous gene model prediction (1); Glycosylation (4); Helix (23); Natural variant (20); Sequence conflict (2); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5THM; |
| Mapped Pubmed ID | 10364826; 10364829; 10368435; 10473775; 10511562; 10545460; 10580988; 10849071; 10978290; 10978302; 110312; 110315; 11063693; 11139506; 11139510; 11164043; 11230529; 11230530; 11267890; 11279826; 11412033; 11766844; 11862451; 11869341; 11875569; 119674; 12196399; 12399389; 12424525; 12468082; 12679097; 12727233; 12733664; 12807774; 1335624; 1385266; 14154502; 14605208; 14616058; 14668383; 1468643; 15133190; 15166145; 15330116; 1560824; 1582561; 1582562; 15833123; 1593643; 15965264; 15972457; 15972847; 1651203; 16547107; 16547641; 16624913; 16955332; 1700427; 17028974; 17248171; 1736311; 17435238; 1747098; 1769565; 17789533; 18174442; 18245358; 18493064; 18560591; 18666829; 19000835; 19343724; 19411605; 19536450; 19812620; 1981760; 20220848; 20333214; 20371351; 21074052; 2108305; 2108864; 2111791; 2124571; 21480662; 2178209; 22198472; 22496733; 22715942; 23071443; 23171073; 23944235; 24043293; 24220963; 2472143; 24865556; 25038463; 2509404; 2517263; 25193493; 25294943; 25425680; 25671424; 26072452; 26109357; 26374124; 26526100; 26594178; 26801178; 27357258; 27558138; 27794539; 2783177; 28393888; 28569019; 29983616; 30372516; 30478906; 30586; 30879640; 31061470; 3128716; 3130292; 33022193; 33827210; 3800957; 414841; 4197378; 4215417; 4217999; 6419721; 6767273; 6814420; 7476143; 7692291; 7705622; 7713409; 7729696; 7818480; 7841458; 7875329; 7877486; 7915261; 8005413; 8005445; 8019574; 8031295; 8045386; 8045397; 8100789; 8119594; 8150295; 8188055; 8274134; 827556; 8276215; 8327503; 8346032; 8349118; 8355602; 8360077; 8375665; 8536969; 8583898; 8583899; 8587111; 8601498; 8754215; 8813053; 8865667; 8878680; 8913769; 8952085; 8957000; 8972083; 8978040; 9071584; 9093857; 9223257; 9321416; 9321417; 9342399; 9402735; 9435945; 9545464; 9549089; 9656489; 9755207; 9819559; |
| Motif | |
| Gene Encoded By | |
| Mass | 61,125 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |