| IED ID | IndEnz0007000005 |
| Enzyme Type ID | catalase000005 |
| Protein Name |
Catalase-peroxidase CP EC 1.11.1.21 Peroxidase/catalase |
| Gene Name | katG BPSL2865 |
| Organism | Burkholderia pseudomallei (strain K96243) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Burkholderia pseudomallei group Burkholderia pseudomallei (Pseudomonas pseudomallei) Burkholderia pseudomallei (strain K96243) |
| Enzyme Sequence | MSNEAKCPFHQAAGNGTSNRDWWPNQLDLSILHRHSSLSDPMGKDFNYAQAFEKLDLAAVKRDLHALMTTSQDWWPADFGHYGGLFIRMAWHSAGTYRTADGRGGAGEGQQRFAPLNSWPDNANLDKARRLLWPIKQKYGRAISWADLLILTGNVALESMGFKTFGFAGGRADTWEPEDVYWGSEKIWLELSGGPNSRYSGDRQLENPLAAVQMGLIYVNPEGPDGNPDPVAAARDIRDTFARMAMNDEETVALIAGGHTFGKTHGAGPASNVGAEPEAAGIEAQGLGWKSAYRTGKGADAITSGLEVTWTTTPTQWSHNFFENLFGYEWELTKSPAGAHQWVAKGADAVIPDAFDPSKKHRPTMLTTDLSLRFDPAYEKISRRFHENPEQFADAFARAWFKLTHRDMGPRARYLGPEVPAEVLLWQDPIPAVDHPLIDAADAAELKAKVLASGLTVSQLVSTAWAAASTFRGSDKRGGANGARIRLAPQKDWEANQPEQLAAVLETLEAIRTAFNGAQRGGKQVSLADLIVLAGCAGVEQAAKNAGHAVTVPFAPGRADASQEQTDVESMAVLEPVADGFRNYLKGKYRVPAEVLLVDKAQLLTLSAPEMTVLLGGLRVLGANVGQSRHGVFTAREQALTNDFFVNLLDMGTEWKPTAADADVFEGRDRATGELKWTGTRVDLVFGSHSQLRALAEVYGSADAQEKFVRDFVAVWNKVMNLDRFDLA |
| Enzyme Length | 728 |
| Uniprot Accession Number | Q939D2 |
| Absorption | |
| Active Site | ACT_SITE 92; /note=Proton acceptor |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Displays also NADH oxidase, isoniazid hydrazine lyase and isonicotinoyl-NAD synthase activity. {ECO:0000255|HAMAP-Rule:MF_01961, ECO:0000269|PubMed:15280362}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5 for the peroxidase reaction, 6.0 for the catalase reaction, and 8.75 for the NADH oxidase reaction. {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (14); Chain (1); Cross-link (2); Erroneous initiation (2); Helix (46); Initiator methionine (1); Metal binding (1); Site (1); Turn (6) |
| Keywords | 3D-structure;Direct protein sequencing;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_01961}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (27) |
| Cross Reference PDB | 5L02; 5L05; 5SW4; 5SW5; 5SW6; 5SX0; 5SX1; 5SX2; 5SX3; 5SX6; 5SX7; 5SXQ; 5SXR; 5SXS; 5SXT; 5SXW; 5SXX; 5SYH; 5SYI; 5SYK; 5SYL; 5SYV; 5SYX; 6CFQ; 6MPY; 6MQ0; 6MQ1; |
| Mapped Pubmed ID | 16618106; 17063492; 20554537; 24568093; 24785434; 28409923; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,446 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=56 mM for H(2)O(2) for the catalase reaction (at pH 5.5-6.0) {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; KM=4.5 mM for H(2)O(2) for the catalase reaction (at pH 7.0) {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; KM=700 uM for H(2)O(2) for the peroxidase reaction {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; KM=300 uM for ABTS for the peroxidase reaction {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; KM=12.5 uM for NADH for the oxidase reaction {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; Vmax=11900 umol/min/mg enzyme for H(2)O(2) for the catalase reaction (at pH 5.5-6.0) {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; Vmax=4300 umol/min/mg enzyme for H(2)O(2) for the catalase reaction (at pH 7.0) {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; Vmax=6.0 umol/min/mg enzyme for ABTS for the peroxidase reaction {ECO:0000269|PubMed:16211084, ECO:0000269|PubMed:18178143}; |
| Metal Binding | METAL 259; /note=Iron (heme b axial ligand) |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda | 1.11.1.21; |