| IED ID | IndEnz0007000006 |
| Enzyme Type ID | catalase000006 |
| Protein Name |
Catalase-peroxidase CP EC 1.11.1.21 Peroxidase/catalase |
| Gene Name | katG sll1987 |
| Organism | Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Synechococcales Merismopediaceae Synechocystis unclassified Synechocystis Synechocystis sp. PCC 6803 Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Enzyme Sequence | MGTQPARKLRNRVFPHPHNHRKEKPMANDQVPASKCPVMHGANTTGQNGNLNWWPNALNLDILHQHDRKTNPMDDGFNYAEAFQQLDLAAVKQDLHHLMTDSQSWWPADWGHYGGLMIRMAWHAAGTYRIADGRGGAATGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKLSWGDLIILAGTMAYESMGLKVYGFAGGREDIWHPEKDIYWGAEKEWLASSDHRYGSEDRESLENPLAAVQMGLIYVNPEGVDGHPDPLCTAQDVRTTFARMAMNDEETVALTAGGHTVGKCHGNSKAELIGPEPEGADVVEQGLGWHNQNGKGVGRETMSSGIEGAWTTHPTQWDNGYFYMLFNHEWELKKSPAGAWQWEPVNIKEEDKPVDVEDPNIRHNPIMTDADMAMIKDPIYRQISERFYREPDYFAEVFAKAWFKLTHRDLGPKSRYLGPDVPQEDLIWQDPIPPVDYTLSEGEIKELEQQILASGLTVSELVCTAWDSARTFRSSDYRGGANGARIRLEPQKNWPGNEPTRLAKVLAVLENIQANFAKPVSLADLIVLGGGAAIAKAALDGGIEVNVPFLPGRGDATQAMTDAESFTPLEPIHDGYRNWLKQDYAVSPEELLLERTQLMGLTAPEMTVLIGGMRVLGTNHGGTKHGVFTDRVGVLSNDFFVNLTDMAYQWRPAGNNLYEIGDRQTGEVKWTATKVDLVFGSNSILRSYAEVYAQDDNREKFVRDFVAAWTKVMNADRFDLPRG |
| Enzyme Length | 754 |
| Uniprot Accession Number | P73911 |
| Absorption | |
| Active Site | ACT_SITE 106; /note="Tryptophan radical intermediate"; /evidence="ECO:0000269|PubMed:16574230, ECO:0000269|PubMed:17260948"; ACT_SITE 123; /note="Proton acceptor"; /evidence="ECO:0000255|HAMAP-Rule:MF_01961" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Displays also NADH oxidase, isoniazid hydrazine lyase and isonicotinoyl-NAD synthase activity. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.25 for the peroxidase reaction and 6.5 for the catalase reaction. {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Cross-link (2); Initiator methionine (1); Metal binding (1); Region (1); Site (1) |
| Keywords | Heme;Hydrogen peroxide;Iron;Metal-binding;Organic radical;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_01961}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16526092; 18000013; |
| Motif | |
| Gene Encoded By | |
| Mass | 84,446 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=20 mM for H(2)O(2) for the catalase reaction (at pH 5.5-6.0) {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; KM=3.1 mM for H(2)O(2) for the catalase reaction (at pH 7.0) {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; KM=1000 uM for H(2)O(2) for the peroxidase reaction {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; KM=7 uM for ABTS for the peroxidase reaction {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; Vmax=6000 umol/min/mg enzyme for H(2)O(2) for the catalase reaction (at pH 5.5-6.0) {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; Vmax=5400 umol/min/mg enzyme for H(2)O(2) for the catalase reaction (at pH 7.0) {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; Vmax=9.3 umol/min/mg enzyme for ABTS for the peroxidase reaction {ECO:0000269|PubMed:10543446, ECO:0000269|PubMed:18178143, ECO:0000269|PubMed:9919646}; |
| Metal Binding | METAL 290; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_01961 |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda | 1.11.1.21; |