| IED ID | IndEnz0007000012 |
| Enzyme Type ID | catalase000012 |
| Protein Name |
Catalase-peroxidase CP EC 1.11.1.21 Peroxidase/catalase |
| Gene Name | katG cpeA AN7388 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MGSNECPYSRQNANIGGGGQNNRDWWPDDLKLNILRQHNSVSNPLDKGFDYTAAFNSLDYFGLKRDLEALMTDSQDWWPADFGHYGGLFIRMAWHSAGTYRVFDGRGGGGQGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGSKISWADLLILAGNVALESMGFKTFGFAGGRSDTWEADQSVFWGGEKEWLGNDVRYLNGELDNPLAASHMGLIYVNPEGPNKNPDPVLAAKDIRITFGRMAMNDEETVALIAGGHTFGKTHGAGPATHLGKEPHGAGIELQGLGWESGFESGTGRHAITSGLEVIWTKTPTKWSNQFFEYLFKYDWELTKSPAGAHQYVAKGVEPFIPDPFDPSIKHPPRMLTTDLSLRYDPEYEKISRRFLENPDQFADAFARAWFKLTHRDVGPRVLYQGPEVPSEVLIWQDPVPPLDHPVIDNDDIATLKKAILNSGISHTDLFSTAWASASTFRGSDKRGGANGARIRLSPQKNWKVNSQPWLSESLAALEKIQKQFNDAQSTDKRVSLADLIVLAGAASLEKAARDAGHNVSVSFTPGRTDATQEQTDVDSFNNLEPIADGFRNYGRGTPRVLTEDFLIDKAQLLNLSPPELTVLIGGLRVLNNNYDRSNLGVFTKRPGQLTNDFFVNLLDMGVQWKPADDTNEIFIGSDRKTGQARWKASRADLVFGSHAELRAISEVYGSSDGEAKFVKDFVAAWEKVSNLDRFDLKQTGLAQRIKPQL |
| Enzyme Length | 739 |
| Uniprot Accession Number | Q96VT4 |
| Absorption | |
| Active Site | ACT_SITE 95; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_03108 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_03108}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_03108}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. {ECO:0000255|HAMAP-Rule:MF_03108}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Cross-link (2); Metal binding (1); Region (1); Site (1) |
| Keywords | Cytoplasm;Direct protein sequencing;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | INDUCTION: Induced by transcription factor stuA upon carbon starvation and during early sexual development. {ECO:0000269|PubMed:12455692}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. Note=Found in sexual structures such as Huelle cells, but not in primordia, hyphae and conidiophores. |
| Modified Residue | |
| Post Translational Modification | PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_03108}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 82,110 |
| Kinetics | |
| Metal Binding | METAL 258; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_03108 |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda |