| IED ID | IndEnz0007000015 |
| Enzyme Type ID | catalase000015 |
| Protein Name |
Catalase HPII EC 1.11.1.6 Hydroxyperoxidase II |
| Gene Name | katE b1732 JW1721 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTPPGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGPTLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKITPVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFVHAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHTFRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEYELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFHPGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHRMGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHPRLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIELTDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALKAKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDANYYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVEADSADGSFMDELLTLMAAHRVWSRIPKIDKIPA |
| Enzyme Length | 753 |
| Uniprot Accession Number | P21179 |
| Absorption | |
| Active Site | ACT_SITE 128; /evidence=ECO:0000255|PROSITE-ProRule:PRU10013; ACT_SITE 201; /evidence=ECO:0000255|PROSITE-ProRule:PRU10013 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10013}; |
| DNA Binding | |
| EC Number | 1.11.1.6 |
| Enzyme Function | FUNCTION: Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (29); Chain (1); Compositional bias (1); Cross-link (1); Helix (36); Metal binding (1); Region (2); Turn (8) |
| Keywords | 3D-structure;Cytoplasm;Direct protein sequencing;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | Itself |
| Induction | INDUCTION: By entry into stationary phase. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (46) |
| Cross Reference PDB | 1CF9; 1GG9; 1GGE; 1GGF; 1GGH; 1GGJ; 1GGK; 1IPH; 1P7Y; 1P7Z; 1P80; 1P81; 1QF7; 1QWS; 1YE9; 3P9P; 3P9Q; 3P9R; 3P9S; 3PQ2; 3PQ3; 3PQ4; 3PQ5; 3PQ6; 3PQ7; 3PQ8; 3TTT; 3TTU; 3TTV; 3TTW; 3TTX; 3VU3; 4BFL; 4ENP; 4ENQ; 4ENR; 4ENS; 4ENT; 4ENU; 4ENV; 4ENW; 5BV2; 6BY0; 6ZTV; 6ZTW; 6ZTX; |
| Mapped Pubmed ID | 10022351; 10488114; 10802173; 11440837; 11763776; 12589799; 12777389; 12916153; 1324193; 1385595; 14980071; 15690043; 15823018; 15915708; 16183297; 16606699; 16858726; 17400245; 21332158; 2187997; 22172685; 2254248; 22820098; 24223139; 24561554; 2977357; 29968670; 33485289; 6094482; |
| Motif | |
| Gene Encoded By | |
| Mass | 84,163 |
| Kinetics | |
| Metal Binding | METAL 415; /note=Iron (heme axial ligand) |
| Rhea ID | RHEA:20309 |
| Cross Reference Brenda |