| IED ID |
IndEnz0007000073 |
| Enzyme Type ID |
catalase000073 |
| Protein Name |
AP-1-like transcription factor napA
|
| Gene Name |
napA ANIA_07513 |
| Organism |
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Pezizomycotina
leotiomyceta
Eurotiomycetes
Eurotiomycetidae
Eurotiales (green and blue molds)
Aspergillaceae
Aspergillus
Aspergillus subgen. Nidulantes
Emericella nidulans (Aspergillus nidulans)
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
|
| Enzyme Sequence |
MADYNSLYQHGLYLSPDQQDLLLAALSSNNPPSKQKQNVQKPELGTNPTNTPGQASTGSFNTSPAFDGSHQFDNLNYDESPFLDFNPELEWDFPGSENLIGELPGSATSDDHEVGEKRKDSNSNGEVNGKKRRESDDKSDDKTSKKPGRKPLTSEPTSKRKAQNRAAQRAFRERKEKHLKDLEAKVEELQKASDSANQENGLLKAQVERLQVELREYRKRLSWVTQGNALSAINSYPGNANRMSGLNNNDFMFDFPKFGDLPGGRIFNGSVAKTNQNKKDDTPIPGILRHSALQAANGRASSSASPKTVTSNNPATKSPVTADGRLTSHTSSVYNYHQPGQGHDTSTSDSPSSSSDSHQFLSSSGTSPEPSVQSPDNQAKESHEGHTCTIDGEKSFCAQLGMACGNINNPIPAVRQRSESATNTPNAPSSTDNVPGIDFMAQQNGGQFDPLLFGDWREPQDAVLSQDFNTFFDDAFPLPDLGSPSHNLTEVGLGAQQKKSILEEMDNKEEEEVVPGEDKAQMLSCTKIWDRLQSMEKFRNGEIDVDNLCSELRTKARCSEGGVVVNQRDVDDIIGRV |
| Enzyme Length |
577 |
| Uniprot Accession Number |
Q5AW17 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
|
| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Transcription activator involved in oxidative stress response, specifically during hyphal growth. Regulates the transcription of genes encoding antioxidant enzymes and components of the cellular thiol-reducing pathways including the mycelium-specific catalase catB (but not the conidia-specific catalase catA), thioredoxin reductase trxB and thioredoxin thiO (PubMed:17617701). Preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3'. Activity of the transcription factor is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Activation by hydroperoxides induces nuclear accumulation and as a result NapA transcriptional activity (By similarity). {ECO:0000250|UniProtKB:P19880, ECO:0000269|PubMed:17617701}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Compositional bias (3); Domain (1); Motif (3); Region (5) |
| Keywords |
Cytoplasm;Nucleus;Reference proteome |
| Interact With |
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| Induction |
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| Subcellular Location |
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P19880}. Cytoplasm {ECO:0000250|UniProtKB:P19880}. Note=Oxidized napA is found predominantly in the nucleus, while reduced napA is continuously exported to the cytoplasm by CRM1/exportin 1. {ECO:0000250|UniProtKB:P19880}. |
| Modified Residue |
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| Post Translational Modification |
PTM: Oxidative stress induces conformational changes through oxidation of cysteine residues, masking the nuclear export signal, thus abolishing nuclear export by CRM1/exportin 1. {ECO:0000250|UniProtKB:P19880}. |
| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
MOTIF 117..124; /note=Bipartite nuclear localization signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00768; MOTIF 144..151; /note=Bipartite nuclear localization signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00768; MOTIF 543..550; /note=Nuclear export signal; /evidence=ECO:0000250|UniProtKB:P19880 |
| Gene Encoded By |
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| Mass |
63,154 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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