| IED ID | IndEnz0007000100 |
| Enzyme Type ID | catalase000100 |
| Protein Name |
Chitooligosaccharide deacetylase ChbG COD EC 3.5.1.105 Chitin disaccharide deacetylase Chitobiose deacetylase Chitobiose-6P deacetylase Chitotriose deacetylase Chitotriose-6P deacetylase |
| Gene Name | chbG ydjC b1733 JW1722 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAIDHAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQELVSQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEQGIALRADRQMAFDLPVNLRTTQGFSSAFYGEEISESLFLQVLDDAGHRGDRSLEVMCHPAFIDNTIRQSAYCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV |
| Enzyme Length | 249 |
| Uniprot Accession Number | P37794 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + N,N'-diacetylchitobiose = acetate + N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine; Xref=Rhea:RHEA:27469, ChEBI:CHEBI:15377, ChEBI:CHEBI:28681, ChEBI:CHEBI:30089, ChEBI:CHEBI:59910; EC=3.5.1.105; Evidence={ECO:0000269|PubMed:22797760}; CATALYTIC ACTIVITY: Reaction=diacetylchitobiose-6'-phosphate + H2O = acetate + N'-monoacetylchitobiose-6'-phosphate; Xref=Rhea:RHEA:35083, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:64883, ChEBI:CHEBI:71315; Evidence={ECO:0000269|PubMed:22797760}; |
| DNA Binding | |
| EC Number | 3.5.1.105 |
| Enzyme Function | FUNCTION: ChbG is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Deacetylation of chitobiose-6-P and chitotriose-6-P is necessary for both the activation of the chb promoter by the regulatory protein ChbR and the hydrolysis of phosphorylated beta-glucosides by the phospho-beta-glucosidase ChbF. Catalyzes the removal of only one acetyl group from chitobiose-6-P to yield monoacetylchitobiose-6-P, the inducer of ChbR and the substrate of ChbF. It can also use chitobiose and chitotriose as substrates. {ECO:0000269|PubMed:22797760}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; chitin degradation. {ECO:0000305|PubMed:9405618}. |
| nucleotide Binding | |
| Features | Chain (1); Metal binding (2); Mutagenesis (3); Sequence conflict (12) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Cytoplasm;Hydrolase;Magnesium;Metal-binding;Polysaccharide degradation;Reference proteome |
| Interact With | |
| Induction | INDUCTION: By N,N'-diacetylchitobiose. {ECO:0000269|PubMed:9405618}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22797760}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16606699; |
| Motif | |
| Gene Encoded By | |
| Mass | 27,774 |
| Kinetics | |
| Metal Binding | METAL 61; /note=Magnesium; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q53WD3; METAL 125; /note=Magnesium; via pros nitrogen; /evidence=ECO:0000250|UniProtKB:Q53WD3 |
| Rhea ID | RHEA:27469; RHEA:35083 |
| Cross Reference Brenda | 3.5.1.105; |