Detail Information for IndEnz0007000117
IED ID IndEnz0007000117
Enzyme Type ID catalase000117
Protein Name Peroxisomal
S
-2-hydroxy-acid oxidase GLO4
EC 1.1.3.15
Glycolate oxidase 4
GOX 4
OsGLO4
Short chain alpha-hydroxy acid oxidase GLO4
Gene Name GLO4 Os07g0616500 B1056G08.112 OsJ_25131
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MEDNLPVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMSTTLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNAIRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEGLMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEAGVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARAVMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGDRIRSLL
Enzyme Length 366
Uniprot Accession Number Q8H3I4
Absorption
Active Site ACT_SITE 255; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P05414
Activity Regulation
Binding Site BINDING 27; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683; BINDING 109; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 132; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683; BINDING 158; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 167; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683; BINDING 231; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 253; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 258; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2; Xref=Rhea:RHEA:16789, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:35179, ChEBI:CHEBI:58123; EC=1.1.3.15; Evidence={ECO:0000250|UniProtKB:Q9LJH5};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16790; Evidence={ECO:0000250|UniProtKB:Q9LJH5};
DNA Binding
EC Number 1.1.3.15
Enzyme Function FUNCTION: Oxidase that catalyzes the oxidation of a broad range of 2-hydroxyacids to the corresponding 2-oxoacids, with a reduction of O2 to H2O2. May be involved in a general medium- and long-chain fatty acid catabolic pathway such as alpha-oxidation. {ECO:0000250|UniProtKB:Q9LJH5}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000250|UniProtKB:Q9LJH5}.
nucleotide Binding NP_BIND 80..82; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; NP_BIND 130..132; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; NP_BIND 286..290; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; NP_BIND 309..310; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414
Features Active site (1); Binding site (8); Chain (1); Domain (1); Frameshift (1); Motif (1); Nucleotide binding (4); Sequence conflict (2)
Keywords FMN;Fatty acid metabolism;Flavoprotein;Lipid metabolism;Oxidoreductase;Peroxisome;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Peroxisome {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 364..366; /note=Microbody targeting signal; /evidence=ECO:0000255
Gene Encoded By
Mass 39,764
Kinetics
Metal Binding
Rhea ID RHEA:16789; RHEA:16790
Cross Reference Brenda