| IED ID | IndEnz0007000117 |
| Enzyme Type ID | catalase000117 |
| Protein Name |
Peroxisomal S -2-hydroxy-acid oxidase GLO4 EC 1.1.3.15 Glycolate oxidase 4 GOX 4 OsGLO4 Short chain alpha-hydroxy acid oxidase GLO4 |
| Gene Name | GLO4 Os07g0616500 B1056G08.112 OsJ_25131 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MEDNLPVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMSTTLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNAIRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEGLMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEAGVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARAVMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGDRIRSLL |
| Enzyme Length | 366 |
| Uniprot Accession Number | Q8H3I4 |
| Absorption | |
| Active Site | ACT_SITE 255; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P05414 |
| Activity Regulation | |
| Binding Site | BINDING 27; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683; BINDING 109; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 132; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683; BINDING 158; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 167; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683; BINDING 231; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 253; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; BINDING 258; /note=2-oxocarboxylate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00683 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2; Xref=Rhea:RHEA:16789, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:35179, ChEBI:CHEBI:58123; EC=1.1.3.15; Evidence={ECO:0000250|UniProtKB:Q9LJH5};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16790; Evidence={ECO:0000250|UniProtKB:Q9LJH5}; |
| DNA Binding | |
| EC Number | 1.1.3.15 |
| Enzyme Function | FUNCTION: Oxidase that catalyzes the oxidation of a broad range of 2-hydroxyacids to the corresponding 2-oxoacids, with a reduction of O2 to H2O2. May be involved in a general medium- and long-chain fatty acid catabolic pathway such as alpha-oxidation. {ECO:0000250|UniProtKB:Q9LJH5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000250|UniProtKB:Q9LJH5}. |
| nucleotide Binding | NP_BIND 80..82; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; NP_BIND 130..132; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; NP_BIND 286..290; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414; NP_BIND 309..310; /note=FMN; /evidence=ECO:0000250|UniProtKB:P05414 |
| Features | Active site (1); Binding site (8); Chain (1); Domain (1); Frameshift (1); Motif (1); Nucleotide binding (4); Sequence conflict (2) |
| Keywords | FMN;Fatty acid metabolism;Flavoprotein;Lipid metabolism;Oxidoreductase;Peroxisome;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 364..366; /note=Microbody targeting signal; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 39,764 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:16789; RHEA:16790 |
| Cross Reference Brenda |