| IED ID | IndEnz0007000198 |
| Enzyme Type ID | catalase000198 |
| Protein Name |
Peroxisomal hydratase-dehydrogenase-epimerase HDE Multifunctional beta-oxidation protein MFP Includes: 2-enoyl-CoA hydratase EC 4.2.1.119 ; 3R -3-hydroxyacyl-CoA dehydrogenase EC 1.1.1.n12 |
| Gene Name | fox-2 NCU08828 |
| Organism | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Sordariaceae Neurospora Neurospora crassa Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
| Enzyme Sequence | MAEQLRFDGQVVVVTGAGGGLGKAYCLFFGSRGASVVVNDLGASFKGEGNSTKAADVVVNEIKAAGGKAVANYDSVENGDKIIETAIKEFGRIDILINNAGILRDISFKNMKDEDWDLIFKVHVKGSYKTARAAWPYFRKQKFGRVINTASAAGLFGNFGQANYSAAKLGMVGFTETLAKEGLKYNIISNVIAPIAASRMTETVMPPDLLALMKPEWVVPLVAVLVHKNNTSETGSIFEVGGGHVAKLRWERSSGLLLKADESYTPGAIIKKWDQVTDFSNPQYPTGPNDFLALLEESLKLGPNDPGEKVDFKGRVALVTGGGAGIGRAYCLAFARAGASVVVNDLVNPDDVVNEIKKMGGKAVGAKFSAEDGDAVVKAAIDAFGRVDIVVNNAGILRDKAFHNMDDSLWDPVMNVHARGTYKVTKAAWPYFLKQKYGRVLNTTSTSGIYGNFGQANYSAAKCAILGFSRAIALEGAKYNIYVNTIAPNAGTAMTKTILPEELVQAFKPDYVAPLVLALCSDKVPKKPTGGLYEVGSGWCGQTRWQRSGGHGFPVDVPLTPEEVVKHWNDIVTFDSRADHPEKASDSIEKIMANMENRVGEGKSGAAENEHLAAIKKFTGVEGKGTEYTFTERDVCLYNLGIGAKRTDIKYIFEGNEDFEVVPTFGVIPPFNTEMPFSFDDIVPNFSPMMLLHGEQYLEVRKYPIPTSGRLVSKGKLLEVVDKGSAAIVKQGITTFNAETGEELFYNEMTVFLRGCGGFGGQKKPADRGASTAANKPPARSPDAVVEVQTTEEQAAIYRLSGDYNPLHVDPAFAKVGGFKVPILHGLCSFGIAGKAVYEKYGKFKNIKVRFAGTVNPGQTLVTEMWKEGNKVVFQTKVKETGKLAISGAAAELA |
| Enzyme Length | 894 |
| Uniprot Accession Number | Q01373 |
| Absorption | |
| Active Site | ACT_SITE 164; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10001; ACT_SITE 458; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10001 |
| Activity Regulation | |
| Binding Site | BINDING 21; /note=NAD; via amide nitrogen; /evidence=ECO:0000250; BINDING 40; /note=NAD; /evidence=ECO:0000250; BINDING 99; /note=NAD; via carbonyl oxygen; /evidence=ECO:0000250; BINDING 151; /note=Substrate; /evidence=ECO:0000250; BINDING 445; /note=Substrate; /evidence=ECO:0000250; BINDING 723; /note=(3R)-3-hydroxydecanoyl-CoA; /evidence=ECO:0000250; BINDING 826; /note=(3R)-3-hydroxydecanoyl-CoA; via amide nitrogen; /evidence=ECO:0000250; BINDING 851; /note=(3R)-3-hydroxydecanoyl-CoA; via carbonyl oxygen; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:26526, ChEBI:CHEBI:15377, ChEBI:CHEBI:57319, ChEBI:CHEBI:58856; EC=4.2.1.119; CATALYTIC ACTIVITY: Reaction=a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH; Xref=Rhea:RHEA:32711, ChEBI:CHEBI:15378, ChEBI:CHEBI:57319, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.n12; |
| DNA Binding | |
| EC Number | 4.2.1.119; 1.1.1.n12 |
| Enzyme Function | FUNCTION: Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Lipid metabolism; fatty acid beta-oxidation. |
| nucleotide Binding | NP_BIND 13..37; /note=NAD; /evidence=ECO:0000250; NP_BIND 75..76; /note=NAD; /evidence=ECO:0000250; NP_BIND 164..168; /note=NAD; /evidence=ECO:0000250; NP_BIND 196..199; /note=NAD; /evidence=ECO:0000250; NP_BIND 318..342; /note=NAD; /evidence=ECO:0000250 |
| Features | Active site (2); Binding site (8); Chain (1); Domain (1); Nucleotide binding (5); Region (5) |
| Keywords | Direct protein sequencing;Fatty acid metabolism;Isomerase;Lipid metabolism;Lyase;Multifunctional enzyme;NAD;Oxidoreductase;Peroxisome;Reference proteome;Repeat |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}. Note=Catalase-free microbodies. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 96,326 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:26526; RHEA:32711 |
| Cross Reference Brenda |