| IED ID | IndEnz0007000350 |
| Enzyme Type ID | catalase000350 |
| Protein Name |
Malate dehydrogenase NADP, chloroplastic EC 1.1.1.82 NADP-MDH |
| Gene Name | At5g58330 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAMAELSTPKTTSPFLNSSSRLRLSSKLHLSNHFRHLLLPPLHTTTPNSKISCSVSQNSQAPVAVQENGLVKTKKECYGVFCLTYDLKAEEETRSWKKLINIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDPNEVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVITDHKWLEEGFTESVQKRGGLLIQKWGRSSAASTAVSIVDAIKSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRCVAHLTGEGIAYCDLGPVDTMLPGEV |
| Enzyme Length | 443 |
| Uniprot Accession Number | Q8H1E2 |
| Absorption | |
| Active Site | ACT_SITE 282; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P11708 |
| Activity Regulation | ACTIVITY REGULATION: Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis. |
| Binding Site | BINDING 187; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P11708; BINDING 193; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P11708; BINDING 200; /note=NADP; /evidence=ECO:0000250|UniProtKB:P11708; BINDING 207; /note=NADP; /evidence=ECO:0000250|UniProtKB:P11708; BINDING 226; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P11708; BINDING 257; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P11708 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(S)-malate + NADP(+) = H(+) + NADPH + oxaloacetate; Xref=Rhea:RHEA:10824, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, ChEBI:CHEBI:16452, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.82; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 1.1.1.82 |
| Enzyme Function | FUNCTION: The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells (Probable). Plays an essential role in the regulation of catalase activity and the accumulation of a hydrogen peroxide-dependent signal by transmitting the redox state of the chloroplast to other cell compartments (PubMed:24591715). {ECO:0000269|PubMed:24591715, ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 106..112; /note=NADP; /evidence=ECO:0000250|UniProtKB:P11708; NP_BIND 224..226; /note=NADP; /evidence=ECO:0000250|UniProtKB:P11708 |
| Features | Active site (1); Alternative sequence (1); Binding site (6); Chain (1); Cross-link (1); Disulfide bond (2); Erroneous initiation (1); Nucleotide binding (2); Sequence conflict (2); Site (2); Transit peptide (1) |
| Keywords | Alternative splicing;Chloroplast;Disulfide bond;Isopeptide bond;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide;Ubl conjugation |
| Interact With | |
| Induction | INDUCTION: By low temperature and high light. {ECO:0000269|PubMed:17925997}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12368493; 12938931; 14576160; 14671022; 15052571; 15200639; 15247402; 15276449; 15352244; 16207701; 16648217; 16998091; 17163439; 17828791; 18298409; 18431481; 18538804; 18633119; 18811951; 26380567; 28627464; 29933514; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,316 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10824 |
| Cross Reference Brenda | 1.1.1.40;1.1.1.82; |