Detail Information for IndEnz0007000420
IED ID IndEnz0007000420
Enzyme Type ID catalase000420
Protein Name Catalase easC
EC 1.11.-.-
Ergot alkaloid synthesis protein C
Gene Name easC MCYG_06051
Organism Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) (Microsporum canis)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Arthrodermataceae (dermatophytes) Microsporum Arthroderma otae (Microsporum canis) Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) (Microsporum canis)
Enzyme Sequence MAPNAADKCPVMNNAAEKCPVMHSNGVSSIQSHGPRDIYTLEALSHFNREKIPERAVHAKGTGAYGEFEVTADISDICNVDMLLGVGKKTQCVTRFSTTGLERGSSDGVRDLKGMAVKFFTEQGDWDWVSLNFPFFFIRDPAKFPDMIHSQRRDPQTNLLNPSMTWDFVTKNPEALHMTLLQHSDFGTMFTWRTLSSYAGHAFKWVMPDGSFKYVHFFLASDRGPNFTDGSTAKIDPNDPDFATKDLFEAIERGDYPTWTANVQVIDPKDAPKLGFNILDITKHWNLGTYPKGLDTIPSRPFGKLTLNRNVKDYFTEVEKLAFSPSNLVPGVEPSEDPILQARMFAYPDAQRYRLGIDHLKAPIRRKETSCKHDLGPEFDQWLSQVTSESWSHPHEDDYKFAREYYEVLPEFRSQEFQDRMVENLYKSVAQGPEDLRKRVYETFELVSTELARRVREGAEVIVADMAQPDSPERAQPGQQRL
Enzyme Length 482
Uniprot Accession Number C5FTM9
Absorption
Active Site ACT_SITE 58; /evidence=ECO:0000250|UniProtKB:P15202
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 1.11.-.-
Enzyme Function FUNCTION: Catalase; part of the gene cluster that mediates the biosynthesis of fungal ergot alkaloid (PubMed:22403186). DmaW catalyzes the first step of ergot alkaloid biosynthesis by condensing dimethylallyl diphosphate (DMAP) and tryptophan to form 4-dimethylallyl-L-tryptophan (PubMed:22403186). The second step is catalyzed by the methyltransferase easF that methylates 4-dimethylallyl-L-tryptophan in the presence of S-adenosyl-L-methionine, resulting in the formation of 4-dimethylallyl-L-abrine (PubMed:22403186). The catalase easC and the FAD-dependent oxidoreductase easE then transform 4-dimethylallyl-L-abrine to chanoclavine-I which is further oxidized by easD in the presence of NAD(+), resulting in the formation of chanoclavine-I aldehyde (PubMed:22403186). Chanoclavine-I aldehyde is the precursor of ergoamides and ergopeptines in Clavicipitaceae, and clavine-type alcaloids such as fumiclavine in Trichocomaceae (PubMed:22403186). However, the metabolites downstream of chanoclavine-I aldehyde in Arthrodermataceae have not been identified yet (PubMed:22403186). {ECO:0000269|PubMed:22403186}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Alkaloid biosynthesis; ergot alkaloid biosynthesis. {ECO:0000305|PubMed:22403186}.
nucleotide Binding
Features Active site (1); Chain (1); Metal binding (1)
Keywords Alkaloid metabolism;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,898
Kinetics
Metal Binding METAL 347; /note=Iron (heme axial ligand); /evidence=ECO:0000250|UniProtKB:P15202
Rhea ID
Cross Reference Brenda