| IED ID | IndEnz0007000440 |
| Enzyme Type ID | catalase000440 |
| Protein Name |
Catalase-related peroxidase EC 1.11.1.- |
| Gene Name | MAP_2744c |
| Organism | Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) (Mycobacterium paratuberculosis) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium avium complex (MAC) Mycobacterium avium Mycobacterium paratuberculosis Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) (Mycobacterium paratuberculosis) |
| Enzyme Sequence | MSGGLTPDQAIDAIRGTGGAQPGCRALHAKGTLYRGTFTATRDAVMLSAAPHLDGSTVPALIRFSNGSGNPKQRDGAPGVRGMAVKFTLPDGSTTDVSAQTARLLVSSTPEGFIDLLKAMRPGLTTPLRLATHLLTHPRLLGALPLLREANRIPASYATTEYHGLHAFRWIAADGSARFVRYHLVPTAAEEYLSASDARGKDPDFLTDELAARLQDGPVRFDFRVQIAGPTDSTVDPSSAWQSTQIVTVGTVTITGPDTEREHGGDIVVFDPMRVTDGIEPSDDPVLRFRTLVYSASVKLRTGVDRGAQAPPV |
| Enzyme Length | 313 |
| Uniprot Accession Number | Q73WB6 |
| Absorption | |
| Active Site | ACT_SITE 28; /evidence=ECO:0000250|UniProtKB:P04040 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.11.1.- |
| Enzyme Function | FUNCTION: Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides. {ECO:0000269|PubMed:19827095}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (13); Chain (1); Helix (11); Metal binding (1) |
| Keywords | 3D-structure;Heme;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 3E4W; 3E4Y; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 33,321 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=30 mM for H(2)O(2) in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) oxidation assay (at pH 7.0) {ECO:0000269|PubMed:19827095}; KM=22 mM for t-butyl hydroperoxide in ABTS oxidation assay (at pH 7.0) {ECO:0000269|PubMed:19827095}; KM=3 mM for cumene hydroperoxide in ABTS oxidation assay (at pH 7.0) {ECO:0000269|PubMed:19827095}; KM=49 uM for 9S-hydroperoxy-octadecadienoic acid (9S-HPODE) used as a cosubstrate with ABTS (at pH 7.0) {ECO:0000269|PubMed:19827095}; Note=kcat is 13 sec(-1) for H(2)O(2). kcat is 320 sec(-1) for t-butyl hydroperoxide. kcat is 330 sec(-1) for cumene hydroperoxide. kcat is 529 sec(-1) for 9S-HPODE. {ECO:0000269|PubMed:19827095}; |
| Metal Binding | METAL 294; /note=Iron (heme axial ligand) |
| Rhea ID | |
| Cross Reference Brenda |