| IED ID | IndEnz0007000450 |
| Enzyme Type ID | catalase000450 |
| Protein Name |
Mitogen-activated protein kinase kinase 1 AtMKK1 MAP kinase kinase 1 EC 2.7.12.2 AtMEK1 NMAPKK |
| Gene Name | MKK1 MEK1 At4g26070 F20B18.180 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MNRGSLCPNPICLPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSFYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDPRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLAN |
| Enzyme Length | 354 |
| Uniprot Accession Number | Q94A06 |
| Absorption | |
| Active Site | ACT_SITE 190; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027" |
| Activity Regulation | ACTIVITY REGULATION: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, abscisic acid (ABA), hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. {ECO:0000269|PubMed:11874576, ECO:0000269|PubMed:15225555, ECO:0000269|PubMed:17059410, ECO:0000269|PubMed:17728292}. |
| Binding Site | BINDING 97; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.12.2; CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.12.2; CATALYTIC ACTIVITY: Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.12.2; |
| DNA Binding | |
| EC Number | 2.7.12.2 |
| Enzyme Function | FUNCTION: MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H(2)O(2) production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. {ECO:0000269|PubMed:10759527, ECO:0000269|PubMed:17059410, ECO:0000269|PubMed:17728292, ECO:0000269|PubMed:18248592, ECO:0000269|PubMed:18599650, ECO:0000269|PubMed:18982020, ECO:0000269|PubMed:19484493}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 74..82; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Features | Active site (1); Alternative sequence (2); Binding site (1); Chain (1); Domain (1); Modified residue (3); Mutagenesis (6); Nucleotide binding (1) |
| Keywords | ATP-binding;Alternative splicing;Immunity;Innate immunity;Kinase;Nucleotide-binding;Phosphoprotein;Plant defense;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase |
| Interact With | Q39008; Q9LMM5; Q8GYQ5; Q39024 |
| Induction | INDUCTION: By wounding. {ECO:0000269|PubMed:9426629}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 218; /note="Phosphothreonine"; /evidence="ECO:0000269|PubMed:11874576, ECO:0000269|PubMed:11875555, ECO:0000269|PubMed:16211390"; MOD_RES 224; /note="Phosphoserine"; /evidence="ECO:0000269|PubMed:11875555, ECO:0000269|PubMed:16211390"; MOD_RES 228; /note="Phosphothreonine"; /evidence="ECO:0000250|UniProtKB:O80397" |
| Post Translational Modification | PTM: Phosphorylation at Thr-218 and Ser-224 by MAP kinase kinase kinases positively regulates kinase activity. {ECO:0000269|PubMed:11874576, ECO:0000269|PubMed:11875555, ECO:0000269|PubMed:16211390}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10526174; 11500556; 17284251; 18650403; 19000167; 19496172; 21046144; 21445343; 21477822; 21798944; 22049531; 22423965; 22643122; 23263767; 23511202; 23695980; 27490954; 28716416; 29440595; 30185442; 30590768; 31187685; 31449235; 31844896; 32165446; 32890733; 9804171; |
| Motif | |
| Gene Encoded By | |
| Mass | 39,210 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17989; RHEA:46608; RHEA:10596 |
| Cross Reference Brenda | 2.7.12.2; |