Detail Information for IndEnz0007000539
IED ID IndEnz0007000539
Enzyme Type ID catalase000539
Protein Name DNA protection during starvation protein
EC 1.16.-.-
Gene Name dps E2348C_0764
Organism Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli O127:H6 Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Enzyme Sequence MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLSLITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKTPLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLWFIESNIE
Enzyme Length 167
Uniprot Accession Number B7UM08
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 Fe(2+) + 2 H(+) + H2O2 = 2 Fe(3+) + 2 H2O; Xref=Rhea:RHEA:48712, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; Evidence={ECO:0000255|HAMAP-Rule:MF_01441};
DNA Binding
EC Number 1.16.-.-
Enzyme Function FUNCTION: During stationary phase, binds the chromosome non-specifically, forming a highly ordered and stable dps-DNA co-crystal within which chromosomal DNA is condensed and protected from diverse damages. It protects DNA from oxidative damage by sequestering intracellular Fe(2+) ion and storing it in the form of Fe(3+) oxyhydroxide mineral, which can be released after reduction. One hydrogen peroxide oxidizes two Fe(2+) ions, which prevents hydroxyl radical production by the Fenton reaction. Dps also protects the cell from UV and gamma irradiation, iron and copper toxicity, thermal stress and acid and base shocks. Also shows a weak catalase activity. {ECO:0000255|HAMAP-Rule:MF_01441}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Metal binding (4)
Keywords Cytoplasm;DNA condensation;DNA-binding;Iron;Iron storage;Metal-binding;Oxidoreductase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-Rule:MF_01441}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 18,695
Kinetics
Metal Binding METAL 51; /note=Iron 1; shared with neighboring subunit; /evidence=ECO:0000255|HAMAP-Rule:MF_01441; METAL 78; /note=Iron 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01441; METAL 82; /note=Iron 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01441; METAL 82; /note=Iron 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01441
Rhea ID RHEA:48712
Cross Reference Brenda