| IED ID | IndEnz0007000589 |
| Enzyme Type ID | catalase000589 |
| Protein Name |
Linolenate 9R-lipoxygenase EC 1.13.11.61 NspLOX |
| Gene Name | all8020 |
| Organism | Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Nostocales Nostocaceae Nostoc unclassified Nostoc Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) |
| Enzyme Sequence | MDLNTYLKLLNLLDSESQKIMLELQAMFSAAGLALRGRGTHTDGIIVKGNLTVLHSSDVPSHSLFTPGKKYDVIFRHANIVGGAKDDALINGRGSAIRIGNIGDDLSKPRLLDLVLNTGEVFGLPTARLYHQFFGSDFHQKSDMLASGSLRRYAVEAALRNPDSFTELYYHTQLCYEWVDSKKKSRYARFRLLNPNQSTEGGLLDDSVEIGPRLVLPRKRGDTREKNYLRNEFRQRLTDGNIVEYVLQAQFRSIEDVAVDCSNIWDPNTYPWLDIAAIVLNQDESENDYYQEIAYNPGNTHYDLKLPNSYSVDDFASLGVSGALVHYFGSIVRAERTQYLYGSKDDLPGKPVYFPLPVTEIPSKRFLFLLEKYNFLTDNSYPSDGEHDKIEALVSAMPTTALDLAVGTTDPTDIPDSYFLERRLNGYNPGAIRESSGQEGWTHELTHNLAKYDIKPGLHFPDFVQCRLFVDKQNGVKLHSIKIDDHEITPCQEQWQYAKRTYLQAEFLSQELKLHLARCHFNIEQYVMAIKRRLAPTHPVRAFINPHLEGLIFINSSAVPKIIGSTGFIPIASMLTQGSIVDVMKNELSKLSYMWNPIADLPRDIPGDLFTPAATAYWELLNNYVEQGLLQPFEDELRTEVNAIQVDELFAELKERSLYSGDQPPKYDSSELKSLLMYIIYHSSFLHSWANFKQYDDAGNPNHVSMGDYSQYDQQTQDKIRFSQRSLTWVLSSIRYNSVAVYGSDLLKQLIREKSSILEPGLPLEDLMMSINI |
| Enzyme Length | 773 |
| Uniprot Accession Number | Q8YK97 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(9Z,12Z,15Z)-octadecatrienoate + O2 = (9R,10E,12Z,15Z)-9-hydroperoxyoctadeca-10,12,15-trienoate; Xref=Rhea:RHEA:31687, ChEBI:CHEBI:15379, ChEBI:CHEBI:32387, ChEBI:CHEBI:63241; EC=1.13.11.61; Evidence={ECO:0000269|PubMed:18031288}; |
| DNA Binding | |
| EC Number | 1.13.11.61 |
| Enzyme Function | FUNCTION: Catalyzes the conversion of alpha-linoleate to (9R,10E,12Z,15Z)-9-hydroperoxyoctadeca-10,12,15-trienoate in oxylipin biosynthesis. Also converts alpha-linoleate to (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate. {ECO:0000269|PubMed:18031288}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-10. {ECO:0000269|PubMed:18031288}; |
| Pathway | PATHWAY: Lipid metabolism; oxylipin biosynthesis. {ECO:0000255|PROSITE-ProRule:PRU00726, ECO:0000269|PubMed:18031288}. |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Metal binding (3) |
| Keywords | Fatty acid biosynthesis;Fatty acid metabolism;Iron;Lipid biosynthesis;Lipid metabolism;Metal-binding;Oxidoreductase;Oxylipin biosynthesis;Plasmid;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pCC7120gamma |
| Mass | 87,860 |
| Kinetics | |
| Metal Binding | METAL 515; /note=Iron; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00726; METAL 520; /note=Iron; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00726; METAL 773; /note=Iron; via carboxylate; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00726 |
| Rhea ID | RHEA:31687 |
| Cross Reference Brenda | 1.13.11.B1; |