Detail Information for IndEnz0007000589
IED ID IndEnz0007000589
Enzyme Type ID catalase000589
Protein Name Linolenate 9R-lipoxygenase
EC 1.13.11.61
NspLOX
Gene Name all8020
Organism Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Nostocales Nostocaceae Nostoc unclassified Nostoc Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Enzyme Sequence MDLNTYLKLLNLLDSESQKIMLELQAMFSAAGLALRGRGTHTDGIIVKGNLTVLHSSDVPSHSLFTPGKKYDVIFRHANIVGGAKDDALINGRGSAIRIGNIGDDLSKPRLLDLVLNTGEVFGLPTARLYHQFFGSDFHQKSDMLASGSLRRYAVEAALRNPDSFTELYYHTQLCYEWVDSKKKSRYARFRLLNPNQSTEGGLLDDSVEIGPRLVLPRKRGDTREKNYLRNEFRQRLTDGNIVEYVLQAQFRSIEDVAVDCSNIWDPNTYPWLDIAAIVLNQDESENDYYQEIAYNPGNTHYDLKLPNSYSVDDFASLGVSGALVHYFGSIVRAERTQYLYGSKDDLPGKPVYFPLPVTEIPSKRFLFLLEKYNFLTDNSYPSDGEHDKIEALVSAMPTTALDLAVGTTDPTDIPDSYFLERRLNGYNPGAIRESSGQEGWTHELTHNLAKYDIKPGLHFPDFVQCRLFVDKQNGVKLHSIKIDDHEITPCQEQWQYAKRTYLQAEFLSQELKLHLARCHFNIEQYVMAIKRRLAPTHPVRAFINPHLEGLIFINSSAVPKIIGSTGFIPIASMLTQGSIVDVMKNELSKLSYMWNPIADLPRDIPGDLFTPAATAYWELLNNYVEQGLLQPFEDELRTEVNAIQVDELFAELKERSLYSGDQPPKYDSSELKSLLMYIIYHSSFLHSWANFKQYDDAGNPNHVSMGDYSQYDQQTQDKIRFSQRSLTWVLSSIRYNSVAVYGSDLLKQLIREKSSILEPGLPLEDLMMSINI
Enzyme Length 773
Uniprot Accession Number Q8YK97
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(9Z,12Z,15Z)-octadecatrienoate + O2 = (9R,10E,12Z,15Z)-9-hydroperoxyoctadeca-10,12,15-trienoate; Xref=Rhea:RHEA:31687, ChEBI:CHEBI:15379, ChEBI:CHEBI:32387, ChEBI:CHEBI:63241; EC=1.13.11.61; Evidence={ECO:0000269|PubMed:18031288};
DNA Binding
EC Number 1.13.11.61
Enzyme Function FUNCTION: Catalyzes the conversion of alpha-linoleate to (9R,10E,12Z,15Z)-9-hydroperoxyoctadeca-10,12,15-trienoate in oxylipin biosynthesis. Also converts alpha-linoleate to (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate. {ECO:0000269|PubMed:18031288}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-10. {ECO:0000269|PubMed:18031288};
Pathway PATHWAY: Lipid metabolism; oxylipin biosynthesis. {ECO:0000255|PROSITE-ProRule:PRU00726, ECO:0000269|PubMed:18031288}.
nucleotide Binding
Features Chain (1); Domain (1); Metal binding (3)
Keywords Fatty acid biosynthesis;Fatty acid metabolism;Iron;Lipid biosynthesis;Lipid metabolism;Metal-binding;Oxidoreductase;Oxylipin biosynthesis;Plasmid;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By Plasmid pCC7120gamma
Mass 87,860
Kinetics
Metal Binding METAL 515; /note=Iron; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00726; METAL 520; /note=Iron; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00726; METAL 773; /note=Iron; via carboxylate; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00726
Rhea ID RHEA:31687
Cross Reference Brenda 1.13.11.B1;