| IED ID | IndEnz0007000717 |
| Enzyme Type ID | catalase000717 |
| Protein Name |
2-imino-3- indol-3-yl propanoate dimerase EC 1.21.98.- Catalase EC 1.11.1.6 Violacein biosynthesis protein VioB |
| Gene Name | vioB CV_3273 |
| Organism | Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Neisseriales Chromobacteriaceae Chromobacterium group Chromobacterium Chromobacterium violaceum Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) |
| Enzyme Sequence | MSILDFPRIHFRGWARVNAPTANRDPHGHIDMASNTVAMAGEPFDLARHPTEFHRHLRSLGPRFGLDGRADPEGPFSLAEGYNAAGNNHFSWESATVSHVQWDGGEADRGDGLVGARLALWGHYNDYLRTTFNRARWVDSDPTRRDAAQIYAGQFTISPAGAGPGTPWLFTADIDDSHGARWTRGGHIAERGGHFLDEEFGLARLFQFSVPKDHPHFLFHPGPFDSEAWRRLQLALEDDDVLGLTVQYALFNMSTPPQPNSPVFHDMVGVVGLWRRGELASYPAGRLLRPRQPGLGDLTLRVSGGRVALNLACAIPFSTRAAQPSAPDRLTPDLGAKLPLGDLLLRDEDGALLARVPQALYQDYWTNHGIVDLPLLREPRGSLTLSSELAEWREQDWVTQSDASNLYLEAPDRRHGRFFPESIALRSYFRGEARARPDIPHRIEGMGLVGVESRQDGDAAEWRLTGLRPGPARIVLDDGAEAIPLRVLPDDWALDDATVEEVDYAFLYRHVMAYYELVYPFMSDKVFSLADRCKCETYARLMWQMCDPQNRNKSYYMPSTRELSAPKARLFLKYLAHVEGQARLQAPPPAGPARIESKAQLAAELRKAVDLELSVMLQYLYAAYSIPNYAQGQQRVRDGAWTAEQLQLACGSGDRRRDGGIRAALLEIAHEEMIHYLVVNNLLMALGEPFYAGVPLMGEAARQAFGLDTEFALEPFSESTLARFVRLEWPHFIPAPGKSIADCYAAIRQAFLDLPDLFGGEAGKRGGEHHLFLNELTNRAHPGYQLEVFDRDSALFGIAFVTDQGEGGALDSPHYEHSHFQRLREMSARIMAQSAPFEPALPALRNPVLDESPGCQRVADGRARALMALYQGVYELMFAMMAQHFAVKPLGSLRRSRLMNAAIDLMTGLLRPLSCALMNLPSGIAGRTAGPPLPGPVDTRSYDDYALGCRMLARRCERLLEQASMLEPGWLPDAQMELLDFYRRQMLDLACGKLSREA |
| Enzyme Length | 998 |
| Uniprot Accession Number | Q9S3V0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.6; Evidence={ECO:0000269|PubMed:17176066, ECO:0000269|PubMed:17925955}; CATALYTIC ACTIVITY: Reaction=2 2-iminio-3-(indol-3-yl)propanoate + H2O2 = 2 H2O + indole-3-pyruvate imine dimer; Xref=Rhea:RHEA:51036, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:59193, ChEBI:CHEBI:133928; Evidence={ECO:0000269|PubMed:17176066, ECO:0000269|PubMed:17925955}; |
| DNA Binding | |
| EC Number | 1.21.98.-; 1.11.1.6 |
| Enzyme Function | FUNCTION: Catalyzes the hydrogen peroxide-dependent dimerization of two L-tryptophan-derived molecules (imine form of indole 3-pyruvate (IPA)), to form an uncharacterized product suggested to be indole-3-pyruvate imine dimer that can spontaneously convert into dichlorochromopyrrolate (CPA). The uncharacterized product is the substrate of VioE. {ECO:0000269|PubMed:17176066, ECO:0000269|PubMed:17925955}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis; violacein biosynthesis. |
| nucleotide Binding | |
| Features | Chain (1); Sequence conflict (7) |
| Keywords | Antibiotic biosynthesis;Multifunctional enzyme;Oxidoreductase;Reference proteome |
| Interact With | |
| Induction | INDUCTION: By N-acylhomoserine lactone (AHL). |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 111,226 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:20309; RHEA:51036 |
| Cross Reference Brenda |